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首页> 外文期刊>Mitochondrial DNA >The complete mitochondrial genome of the flesh fly, Sarcophaga impatiens Walker (Diptera: Sarcophagidae)
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The complete mitochondrial genome of the flesh fly, Sarcophaga impatiens Walker (Diptera: Sarcophagidae)

机译：果蝇的完整线粒体基因组，Sarcophaga impatiens Walker（双翅目：Sarcophagidae）

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摘要

Approximately 2500 fly species comprise the Sarcophagidae family worldwide. The complete mitochondrial genome of the carrion-breeding, forensically important Sarcophaga impatiens Walker (Diptera: Sarcophagidae) from Australia was sequenced. The 15,169 bp circular genome contains the 37 genes found in a typical Metazoan genome: 13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes. It also contains one non-coding A+T-rich region. The arrangement of the genes was the same as that found in the ancestral insect. All the protein initiation codons are ATN, except for cox1 that begins with TCG (encoding S). The 22 tRNA anticodons of S. impatiens are consistent with those observed in Drosophila yakuba, and all form the typical cloverleaf structure, except for tRNA-Ser ~(AGN) that lacks the DHU arm. The mitochondrial genome of Sarcophaga presented will be valuable for resolving phylogenetic relationships within the family Sarcophagidae and the order Diptera, and could be used to identify favourable genetic markers for species identifications for forensic purposes.
机译：全球约有2500种蝇类组成石棺科。测序了来自澳大利亚的腐肉繁殖，法医学上重要的Sarcophaga impatiens Walker（Diptera：Sarcophagidae）的完整线粒体基因组。 15169 bp的环状基因组包含在典型的后生动物基因组中发现的37个基因：13个蛋白质编码基因，2个核糖体RNA基因和22个转移RNA基因。它还包含一个非编码的A + T丰富区域。基因的排列与祖先昆虫中发现的相同。除以TCG开头的cox1（编码S）外，所有蛋白质起始密码子均为ATN。凤仙链球菌的22个tRNA反密码子与在果蝇yakuba中观察到的密码子一致，并且除了缺乏DHU臂的tRNA-Ser〜（AGN）以外，均形成典型的苜蓿叶结构。提出的石棺线粒体基因组对于解决石棺科和双翅目内的系统发育关系将是有价值的，并可用于鉴定有利的遗传标记物以进行法医鉴定。

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