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首页> 外文期刊>Cancer letters >Taxol induces caspase-independent cytoplasmic vacuolization and cell death through endoplasmic reticulum (ER) swelling in ASTC-a-1 cells.
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Taxol induces caspase-independent cytoplasmic vacuolization and cell death through endoplasmic reticulum (ER) swelling in ASTC-a-1 cells.

机译：紫杉醇通过ASTC-a-1细胞内质网（ER）肿胀诱导不依赖胱天蛋白酶的细胞质空泡化和细胞死亡。

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摘要

High concentration of taxol was found to induce programmed cell death (PCD) and cytoplasm vacuolization in human lung adenocarcinoma (ASTC-a-1) cells. To elucidate the relationship between the PCD and cytoplasm vacuolization, confocal fluorescence microscopy was performed on the cytoplasm vacuolization, endoplasmic reticulum (ER) and mitochondria swelling after taxol treatment in living cells. erRFP plasmid was used to probe the ER distribution, and SCAT3 plasmid was used to monitor the caspase-3 activation in living cells. Our results showed that taxol induced concentration-dependent and caspases-independent cytoplasm vacuolization and cell death through ER and mitochondria swelling. Live confocal imaging of ASTC-a-1 cells stably expressing SCAT3 further verified that taxol-induced cytoplasm vacuolization and cell death was caspase-3-independent. In conclusion, we found for the first time that taxol induces a paraptosis-like PCD in the ASTC-a-1 cells by cytoplasm vacuolization due to the swelling of both ER and mitochondria without activating the caspase enzymes.
机译：发现高浓度的紫杉醇在人肺腺癌（ASTC-a-1）细胞中诱导程序性细胞死亡（PCD）和细胞质空泡化。为了阐明PCD与细胞质空泡化之间的关系，共聚焦荧光显微镜对活细胞中紫杉醇处理后的细胞质空泡化，内质网（ER）和线粒体肿胀进行了研究。 erRFP质粒用于探测ER分布，SCAT3质粒用于监测活细胞中caspase-3的激活。我们的结果表明，紫杉醇可通过ER和线粒体肿胀诱导浓度依赖性和半胱氨酸蛋白酶依赖性细胞质空泡化和细胞死亡。稳定表达SCAT3的ASTC-a-1细胞的实时共聚焦成像进一步证实了紫杉醇诱导的细胞质空泡化和细胞死亡与caspase-3无关。总之，我们首次发现紫杉醇通过ER和线粒体的溶胀而不激活caspase酶，通过细胞质空泡诱导ASTC-a-1细胞中的类病毒样PCD。

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