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首页> 外文会议>Conference on Biophotonics and Immune Responses; 20080121; San Jose,CA(US) >Fluorescence Imaging Analysis of Taxol-Induced ASTC-a-1 Cell Death with Cell Swelling and Cytoplasmic Vacuolization
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Fluorescence Imaging Analysis of Taxol-Induced ASTC-a-1 Cell Death with Cell Swelling and Cytoplasmic Vacuolization

机译：紫杉醇诱导的ASTC-a-1细胞死亡与细胞肿胀和细胞质空泡化的荧光成像分析。

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摘要

Taxol (Paclitaxel), an isolated component from the bark of the Pacific yew Taxus brevifolia, exhibits a broad spectrum of clinical activity against human cancers. Taxol can promote microtubule (MT) assembly, inhibit depolymerization, and change MT dynamics, resulting in disruption of the normal reorganization of the microtubule network required for mitosis and cell proliferation. However, the molecular mechanism of taxol-induced cell death is still unclear. In this report, CCK-8 was used to assay the inhibition of taxol on the human lung adenocarcinoma (ASTC-a-1) cells viability , confocal fluorescence microscope was used to monitor the morphology changes of cells with taxol treatment. We for the first time describe the characteristics of taxol-induced cells swelling, cytoplasmic vacuolization and cell death. Taxol induced swelling, cytoplasmatic vacuolization and cell death without cell shrinkage and membrane rupture. These features differ from those of apoptosis and resemble the paraptosis, a novel nonapoptotic PCD.
机译：紫杉醇（紫杉醇），是从紫杉紫杉树皮的树皮中分离出来的一种成分，具有广泛的抗人类癌症的临床活性。紫杉酚可以促进微管（MT）组装，抑制解聚并改变MT动态，从而破坏有丝分裂和细胞增殖所需的微管网络的正常重组。但是，紫杉醇诱导的细胞死亡的分子机制仍不清楚。在本报告中，CCK-8用于测定紫杉醇对人肺腺癌（ASTC-a-1）细胞活力的抑制作用，共聚焦荧光显微镜用于监测紫杉醇处理后细胞的形态变化。我们首次描述了紫杉醇诱导的细胞肿胀，胞浆空泡和细胞死亡的特征。紫杉醇诱导肿胀，细胞质空泡化和细胞死亡，而没有细胞收缩和膜破裂。这些特征不同于凋亡的特征，并且类似于新的非凋亡PCD截瘫。

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