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首页> 外文会议>Conference on Biophotonics and Immune Responses >Fluorescence Imaging Analysis of Taxol-Induced ASTC-a-1 Cell Death with Cell Swelling and Cytoplasmic Vacuolization
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Fluorescence Imaging Analysis of Taxol-Induced ASTC-a-1 Cell Death with Cell Swelling and Cytoplasmic Vacuolization

机译：紫杉醇诱导的ASTC-A-1细胞死亡荧光成像分析，细胞溶胀和细胞质真空溶液

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摘要

Taxol (Paclitaxel), an isolated component from the bark of the Pacific yew Taxus brevifolia, exhibits a broad spectrum of clinical activity against human cancers. Taxol can promote microtubule (MT) assembly, inhibit depolymerization, and change MT dynamics, resulting in disruption of the normal reorganization of the microtubule network required for mitosis and cell proliferation. However, the molecular mechanism of taxol-induced cell death is still unclear. In this report, CCK-8 was used to assay the inhibition of taxol on the human lung adenocarcinoma (ASTC-a-1) cells viability , confocal fluorescence microscope was used to monitor the morphology changes of cells with taxol treatment. We for the first time describe the characteristics of taxol-induced cells swelling, cytoplasmic vacuolization and cell death. Taxol induced swelling, cytoplasmatic vacuolization and cell death without cell shrinkage and membrane rupture. These features differ from those of apoptosis and resemble the paraptosis, a novel nonapoptotic PCD.
机译：紫杉醇（紫杉醇）是来自太平洋红豆杉的树皮的孤立组成部分，Brevifolia的孤立组成部分，对人类癌症的广泛临床活动展示了广泛的临床活动。紫杉醇可以促进微管（MT）组装，抑制解聚，改变MT动力学，导致丝分裂和细胞增殖所需的微管网络的正常重组破坏。然而，紫杉醇诱导的细胞死亡的分子机制仍然不清楚。在本报告中，CCK-8用于检测紫杉醇对人肺腺癌（ASTC-A-1）细胞活力的抑制，共聚焦荧光显微镜用于监测紫杉醇治疗细胞的形态变化。我们首次描述紫杉醇诱导细胞溶胀，细胞质液泡和细胞死亡的特征。紫杉醇诱导肿胀，细胞质真空和细胞死亡而无细胞收缩和膜破裂。这些特征与细胞凋亡的不同不同，并且一种新型非凋亡PCD。

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