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首页> 外文期刊>Molecular medicine reports >Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy
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Inflammatory cytokine expression following the use of bipolar electrocoagulation, ultracision harmonic scalpel and cold knife biopsy

机译：使用双极电凝术，超速谐波手术刀和冷刀活检后的炎症细胞因子表达

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摘要

Electrical surgical devices may determine tissue damage through lateral thermal spread and activation of inflammatory processes. Several tissue effects are associated with the use of different surgical instruments. The aim of the present study was to compare tissue damage following the application of cold knife biopsy, bipolar electrocoagulation and the ultracision harmonic scalpel, through the analysis of inflammatory gene mediator expression. Three fragments of the round ligament (length 0.5 cm) were obtained from 22 females who had undergone total or subtotal laparoscopic hysterectomy using three different modes of resection: Cold knife biopsy, bipolar electrocoagulation and ultracision harmonic scalpel. The tissue fragments were examined by quantitative polymerase chain reaction (qPCR) analysis of selected cytokines. Gene expression analysis demonstrated large standard deviations due to individual variability among patients and indicated variability in the concentrations of cytokines in the three different samples. The quantity of cytokine mRNA in the cold knife biopsy samples was generally greater than those obtained by other techniques. Tumor necrosis factor-alpha expression was significantly higher in the sample obtained with the ultracision harmonic scalpel and bipolar electrocoagulation (P=0.033) when compared with cold knife biopsy. The inflammatory response was analyzed by the quantification of gene expression through the use of qPCR. The ultracision harmonic scalpel and bipolar electrocoagulation triggered the inflammatory cascade and resulted in an increased production of cytokines compared with cold knife biopsy.
机译：电外科手术设备可以通过侧向热扩散和炎症过程的激活来确定组织损伤。多种组织效应与不同手术器械的使用有关。本研究的目的是通过分析炎症基因介体的表达，比较冷刀活检，双极电凝和超精密谐波手术刀后的组织损伤。使用三种不同的切除方式：冷刀活检，双极电凝和超精密切开手术刀，从22例接受了全腹腔镜或全次腹腔镜子宫切除术的女性中获得了三个圆形韧带碎片（长度为0.5 cm）。通过选择的细胞因子的定量聚合酶链反应（qPCR）分析检查组织片段。基因表达分析表明，由于患者之间的个体差异而导致较大的标准差，并表明了三种不同样品中细胞因子浓度的差异。冷刀活检样本中细胞因子mRNA的数量通常大于通过其他技术获得的数量。与冷刀活检相比，在使用超精密谐波手术刀和双极电凝术获得的样品中，肿瘤坏死因子-α的表达明显更高（P = 0.033）。通过使用qPCR定量基因表达来分析炎症反应。与冷刀活检相比，超精密谐波手术刀和双极电凝引发了炎症级联反应，并导致细胞因子的产生增加。

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