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首页> 外文期刊>Molecular urology >p53 Mutation in bladder cancer patients in Japan and inhibition of growth by in vitro adenovirus-mediated wild-type p53 transduction in bladder cancer cells.
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p53 Mutation in bladder cancer patients in Japan and inhibition of growth by in vitro adenovirus-mediated wild-type p53 transduction in bladder cancer cells.

机译：日本膀胱癌患者中的p53突变和体外腺病毒介导的野生型p53膀胱癌细胞转导抑制生长。

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BACKGROUND: Altered expression of p53 has been described in nearly half of bladder cancers, and p53 mutations are presumed to play a role in the multistep progression of these tumors. MATERIALS AND METHODS: The incidence of mutation in the p53 gene and its correlation with histopathologic findings and patient survival were evaluated in 105 Japanese patients with bladder cancer. Laboratory experiments were also performed to confirm the infectivity and efficacy in tumor growth inhibition of an adenovirus expressing wild-type p53 in EJ bladder cancer cells. RESULTS: Mutations of p53 were observed in 38 bladder cancer specimens (36%), with a significantly higher incidence of mutation being seen in tumors of higher stage and grade. The overall survival was worse in patients with the p53 mutation. In laboratory experiments, adenoviral vectors infected bladder cancer cells in a dose- and cell density-dependent manner. The adenovirus-mediated transduction of wild-type p53 resulted in dose-dependent growth inhibition of bladder cancer cells in vitro. No significant cytotoxicity was observed after infection by a control adenovirus. CONCLUSION: Transduction of wild-type p53 might be a potential therapeutic option for bladder cancer.
机译：背景：已经在近一半的膀胱癌中描述了p53表达的改变，并且据推测p53突变在这些肿瘤的多步进展中起作用。材料与方法：在日本的105例膀胱癌患者中评估了p53基因突变的发生率及其与组织病理学发现和患者生存的相关性。还进行实验室实验以证实在EJ膀胱癌细胞中表达野生型p53的腺病毒的感染性和抑制肿瘤生长的功效。结果：在38例膀胱癌标本中发现了p53突变（36％），在更高分期和等级的肿瘤中发现了更高的突变发生率。患有p53突变的患者的总生存期较差。在实验室实验中，腺病毒载体以剂量和细胞密度依赖性方式感染膀胱癌细胞。腺病毒介导的野生型p53的转导在体外导致剂量依赖性生长抑制膀胱癌细胞。对照腺病毒感染后未观察到明显的细胞毒性。结论：野生型p53的转导可能是膀胱癌的潜在治疗选择。

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