Frequency of HPRT mutant lymphocytes in a human control population as determined by the T-cell cloning procedure

Edith Huttner; Bettina Holzapfel; Siegfried Kropf

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Frequency of HPRT mutant lymphocytes in a human control population as determined by the T-cell cloning procedure

机译：通过T细胞克隆程序确定的人类对照人群中HPRT突变淋巴细胞的频率

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the T-cell-cloning assay was established to determine the frequency of hypoxanthinc-guanine phosphoribosyl-transferase (HPRT) mutant lymphocytes in the presence of the selective" agent 6-thioguanine in peripheral blood from a human control population.We investigated 44 healthy adults (blood donors) and found a mean mutant frequency of 7.2X 10~(-6) (geometric mean 5.6 X 10~(-6)). An elevated mean mutant frequency occurred in smokers as compared to non-smokers. However, a statistically significant increase was only observed between female smokers and female non-smokers while there was only a slight difference in.the male group. A significant difference in mutant frequency coulcl.be found between individuals younger than 35 years and those above 35, But the difference of the, mutant frequency with age showed up only among smokers. No significant effect of the gender was observed. Mutant frequency was .inversely related to the cloning efficiency.

机译：建立了T细胞克隆试验，以测定人类对照人群外周血中存在选择性“ 6-硫鸟嘌呤”药物时次黄嘌呤-鸟嘌呤磷酸核糖基转移酶（HPRT）突变淋巴细胞的频率。我们调查了44位健康成年人（献血者），发现平均突变频率为7.2X 10〜（-6）（几何平均值为5.6 X 10〜（-6）），与非吸烟者相比，吸烟者的平均突变频率更高。仅在女性吸烟者和女性非吸烟者之间观察到统计学上的显着增加，而男性组则仅有细微的差别;在35岁以下和35岁以上的个体之间，突变频率coulcl有显着差异。突变频率随年龄的变化仅在吸烟者中出现，未观察到性别的显着影响，突变频率与克隆效率成反比。

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《Mutation Research, C. Mutation Research Letters》 |1995年第2期|共9页
作者
Edith Huttner; Bettina Holzapfel; Siegfried Kropf;
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正文语种 eng
中图分类遗传学;
关键词
Human T-lymphocyte; HPRT-genc; Mutant frequency; In vivo; Control population;

机译：人T淋巴细胞;HPRT基因;突变频率;体内;对照组;

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1. Frequency of HPRT mutant lymphocytes in a human control population as determined by the T-cell cloning procedure [J] . Edith Huttner, Bettina Holzapfel, Siegfried Kropf Mutation Research, C. Mutation Research Letters . 1995,第2期

机译：通过T细胞克隆程序确定的人类对照人群中HPRT突变淋巴细胞的频率
2. Mutant frequency at the hprt locus in human lymphocytes in a case-control study of lung cancer. [J] . Cheng TJ, Christiani DC, Liber HL, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 1995,第1a2期

机译：在肺癌的病例对照研究中，人类淋巴细胞hprt位点的突变频率。
3. Studies on the HPRT mutant frequency in T lymphocytes from healthy Indian male population as a function of age and smoking. [J] . Vivek Kumar PR, Zareena Hamza V, Mohankumar MN, Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects . 2004,第1a2期

机译：研究印度健康男性人口的T淋巴细胞中HPRT突变体频率与年龄和吸烟的关系。
4. DETECTION AND COMPARISON OF SOMATIC MUTANTS INDUCED BY γ-RAY AND B(a)P AT HGPRT LOCUS IN HUMAN LYMPHOCYTES [C] . Xu Honglan, Cao Yi, Duan Zhikai, International symposium on nuclear energy and the environment;NEE; 19961014-18;19961014-18; Beijing(CN);Beijing(CN) . 1996

机译：γ射线和B（a）P在人淋巴细胞HGPRT部位引起的体细胞突变的检测与比较
5. An increase in the frequency of hprt mutant T lymphocytes in the peripheral blood, synovial fluid, and synovial tissue of rheumatoid arthritis patients. [D] . Cannons, Jennifer. 1997

机译：类风湿性关节炎患者外周血，滑液和滑膜组织中hprt突变T淋巴细胞的频率增加。
6. A comparison of two techniques for the molecular tracking of specific T-cell responses; CD4+ human T-cell clones persist in a stable hierarchy but at a lower frequency than clones in the CD8+ population. [O] . M K Maini, L R Wedderburn, F C Hall, 1998

机译：分子追踪特定T细胞反应的两种技术的比较； CD4 +人类T细胞克隆以稳定的层次结构持续存在但频率低于CD8 +群体中的克隆。
7. T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood. [O] . Albertini, R J, Castle, K L, Borcherding, W R 1982

机译：T细胞克隆可检测人外周血中存在的对6硫代鸟嘌呤耐药的突变型淋巴细胞。
8. Quantification and Molecular Characterization of 'hprt' Mutants of Human T-Lymphocytes [R] . Moore, M. M., Harrington-Brock, K., Zimmerman, L. J., 1993

机译：人类T淋巴细胞'hprt'突变体的定量和分子特征

Frequency of HPRT mutant lymphocytes in a human control population as determined by the T-cell cloning procedure

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