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首页> 外文期刊>Mutation Research: International Journal on Mutagenesis, Chromosome Breakage and Related Subjects >Synergistic induction of hydroxyl radical-induced DNA single-strand breaks by chromium(VI) compound and cigarette smoke solution.
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Synergistic induction of hydroxyl radical-induced DNA single-strand breaks by chromium(VI) compound and cigarette smoke solution.

机译：铬（VI）化合物和香烟烟雾溶液协同诱导羟自由基诱导的DNA单链断裂。

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摘要

Chromium(VI) compounds and cigarette smoke are known human carcinogens. We found that K2Cr2O7 and cigarette smoke solution synergistically induced DNA single-strand breaks (0.23+/-0.04 breaks per DNA molecule) in pUC118 plasmid DNA. K2Cr2O7 alone or cigarette smoke solution alone induced much less strand breaks (0.03+/-0.01 or 0.07+/-0.02 breaks per DNA molecule, respectively). The synergistic effect was prevented by catalase and by hydroxyl radical scavengers such as deferoxamine, dimethylsulfoxide, d-mannitol, and Tris, but not by superoxide dismutase. Ascorbic acid enhanced the synergism. Glutathione inhibited strand breakage only at high concentrations. Electron spin resonance (ESR) studies using a hydroxyl radical trap demonstrated that hydroxyl radicals were generated when DNA was incubated with K2Cr2O7 and cigarette smoke solution. Hydroxyl radical adduct decreased dose-dependently when strand breakage was prevented by catalase, deferoxamine, dimethylsulfoxide, d-mannitol or Tris, but not significantly by superoxide dismutase. We also used ESR spectroscopy to study the effects of different concentration of ascorbic acid and glutathione. The results showed that hydroxyl radical, which is proposed as a main carcinogenic mechanism for both chromium(VI) compounds and cigarette smoke solution was mainly responsible for the DNA breaks they induced. Copyright 1999 Elsevier Science B.V.
机译：铬（VI）化合物和香烟烟雾是已知的人类致癌物。我们发现K2Cr2O7和香烟烟雾溶液在pUC118质粒DNA中协同诱导DNA单链断裂（每个DNA分子0.23 +/- 0.04断裂）。单独的K2Cr2O7或单独的香烟烟雾溶液引起的链断裂要少得多（每个DNA分子分别断裂0.03 +/- 0.01或0.07 +/- 0.02）。过氧化氢酶和去铁胺，二甲亚砜，d-甘露醇和Tris等羟自由基清除剂可防止协同作用，但超氧化物歧化酶则不能。抗坏血酸增强了协同作用。谷胱甘肽仅在高浓度时抑制链断裂。使用羟基自由基阱的电子自旋共振（ESR）研究表明，将DNA与K2Cr2O7和香烟烟雾溶液一起孵育时会生成羟基自由基。当过氧化氢酶，去铁胺，二甲亚砜，d-甘露醇或Tris阻止链断裂时，羟自由基加合物的剂量依赖性降低，而超氧化物歧化酶则无明显作用。我们还使用ESR光谱研究了不同浓度的抗坏血酸和谷胱甘肽的影响。结果表明，被认为是六价铬化合物和香烟烟雾溶液的主要致癌机理的羟基自由基主要是由它们引起的DNA断裂。版权所有1999 Elsevier Science B.V.

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