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Extensive Degradation of RNA Precursors by the Exosome in Wild-Type Cells

机译：外来体在野生型细胞中RNA前体的广泛降解。

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摘要

The exosome is a complex involved in the maturation of rRNA and sn-snoRNA, in the degradation of short-lived noncoding RNAs, and in the quality control of RNAs produced in mutants. It contains two catalytic subunits, Rrp6p and Dis3p, whose specific functions are not fully understood. We analyzed the transcriptome of combinations of Rrp6p and Dis3p catalytic mutants by high-resolution tiling arrays. We show that Dis3p and Rrp6p have both overlapping and specific roles in degrading distinct classes of substrates. We found that transcripts derived from more than half of intron-containing genes are degraded before splicing. Surprisingly, we also show that the exosome degrades large amounts of tRNA precursors despite the absence of processing defects. These results underscore the notion that large amounts of RNAs produced in wild-type cells are discarded before entering functional pathways and suggest that kinetic competition with degradation proofreads the efficiency and accuracy of processing.
机译：外泌体是一种复杂的复合物，涉及rRNA和sn-snoRNA的成熟，短期非编码RNA的降解以及突变体中产生的RNA的质量控制。它包含两个催化亚基，Rrp6p和Dis3p，其具体功能尚不完全清楚。我们通过高分辨率平铺阵列分析了Rrp6p和Dis3p催化突变体组合的转录组。我们表明，Dis3p和Rrp6p在降解不同类别的底物时具有重叠和特定的作用。我们发现，来自一半以上含内含子基因的转录本在剪接之前已被降解。出人意料的是，我们还显示，尽管没有加工缺陷，外泌体仍能降解大量的tRNA前体。这些结果强调了在野生型细胞中产生的大量RNA在进入功能性途径之前被丢弃的观点，并表明具有降解的动力学竞争证明了加工的效率和准确性。

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