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首页> 外文期刊>Mycologia >Microscopic detection of phosphatase activity of saprophytic andarbuscular mycorrhizal fungi using a fluorogenic substrate
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Microscopic detection of phosphatase activity of saprophytic andarbuscular mycorrhizal fungi using a fluorogenic substrate

机译：使用荧光底物显微镜检测腐生和丛枝菌根真菌的磷酸酶活性

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摘要

The location and activity of saprophytic and arbuscular mycorrhizal (NM) fungal phosphatases have been investigated with a fluorogenic substrate (ELF) that forms a fluorescent crystalline precipitate at the site of phosphatase activity. This ELF substrate method was compared with other detection methods such as fast blue RR salt staining and p-nitrophenyl phosphate. The ELF substrate was successfully used to determine the location of phosphatase activity in AM fungal hyphae, and to visualize the active AM fungal colonization of roots. The substrate was taken up by fungal hyphae, when acid and alkaline buffers were used, and internal phosphatase activity was visualized. The release of both acid and alkaline phosphatases by a saprophytic fungus to an agar medium was demonstrated. ELF combined with an alkaline buffer led to specific staining of active fungal structures such as hyphae, arbuscules and young vesicles in AM-colonized roots. The results indicate that the ELF substrate is a sensitive indicator for fungal phosphatase activity and that it can be used to visualize hyphal phosphatase activity in both roots and external hyphae.
机译：已经用荧光底物（ELF）研究了腐生和丛枝菌根（NM）真菌磷酸酶的位置和活性，该底物在磷酸酶活性位点形成了荧光晶体沉淀。将该ELF底物方法与其他检测方法（例如，快速蓝RR盐染色和对硝基苯基磷酸酯）进行了比较。 ELF底物已成功用于确定AM真菌菌丝中磷酸酶活性的位置，并可视化了根部的主动AM真菌定植。当使用酸和碱缓冲液时，底物被真菌菌丝吸收，并且可见内部磷酸酶活性。证明了腐生真菌将酸性和碱性磷酸酶释放到琼脂培养基中。 ELF与碱性缓冲液的结合导致AM菌根中活性真菌结构（如菌丝，丛枝和幼小囊泡）的特异性染色。结果表明，ELF底物是真菌磷酸酶活性的敏感指示剂，可用于可视化根和外部菌丝中的菌丝磷酸酶活性。

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