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首页> 外文期刊>Mycologia >Immunocytochemical and cytochemical localization of chitinase and chitin in the infected hosts of a biotrophic mycoparasite, Piptocephalis virginiana.
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Immunocytochemical and cytochemical localization of chitinase and chitin in the infected hosts of a biotrophic mycoparasite, Piptocephalis virginiana.

机译：几丁质酶和几丁质在生物营养性真菌寄生虫，Piptocephalis virginiana的感染宿主中的免疫细胞化学和细胞化学定位。

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摘要

Localization of chitinase and chitin in the uninfected and infected mucoraceous hosts (Choanephora cucurbitarum and Phascolomyces articulosus) of the mycoparasite P. virginiana, was investigated immunocytochemically with an antibody raised against purified chitinase, and cytochemically with wheat germ lectin for the detection of N-acetyl-D-glucosamine oligomers. In the uninfected susceptible host (C. cucurbitarum) chitinase occurred in cytoplasmic vacuoles, and in the resistant host (P. articulosus) chitinase occurred in the cell wall and the vacuoles. In both hosts, infection by P. virginiana destroyed vacuolar integrity and the chitinase was dispersed into the cytoplasm. Accumulation of chitinase only occurred in P. articulosus (at the penetration sites). N-acetyl-D-glucosamine oligomers occurred in the cell wall of both host fungi and in P. articulosus and its haustorium. In infected P. articulosus, papillae and the extrahaustorial matrix contained N-acetyl-D-glucosamine, suggesting cell wall-like composition. It is concluded that differences in the spatial localization of chitinases and N-acetyl-D-glucosamine oligomers in the hosts are related to their compatible and incompatible interactions with P. virginiana.
机译：几丁质酶和几丁质在未感染和感染的粘液寄生性维尔氏疟原虫粘膜衣原体宿主（Choanephora cucurbitarum和Phascolomyces articulosus）中的定位，用针对纯化几丁质酶的抗体进行了免疫细胞化学研究，并用小麦胚芽凝集素进行了细胞化学检测N-乙酰基-D-葡萄糖胺低聚物。在未感染的易感宿主（葫芦丝C. cucurbitarum）中，几丁质酶发生在细胞质液泡中，而在耐药宿主（P. articulosus）中，几丁质酶发生在细胞壁和液泡中。在这两个宿主中，弗吉尼亚假单胞菌的感染破坏了液泡完整性，并且几丁质酶分散到细胞质中。几丁质酶的积累仅发生在P. articulosus中（在穿透位点）。 N-乙酰基-D-葡糖胺低聚物同时存在于宿主真菌的细胞壁和青蒿假单胞菌及其吸虫的细胞壁中。在被感染的P. articulosus中，乳头和胃外基质含有N-乙酰基-D-葡萄糖胺，提示细胞壁样组成。结论是，几丁质酶和N-乙酰基-D-葡萄糖胺低聚物在宿主中的空间定位差异与它们与弗吉尼亚假单胞菌的相容和不相容相互作用有关。

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