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首页> 外文期刊>Mycologia >PHYLOGENETIC RELATIONSHIPS WITHIN SARCOSCYPHA BASED UPON NUCLEOTIDE SEQUENCES OF THE INTERNAL TRANSCRIBED SPACER OF NUCLEAR RIBOSOMAL DNA
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PHYLOGENETIC RELATIONSHIPS WITHIN SARCOSCYPHA BASED UPON NUCLEOTIDE SEQUENCES OF THE INTERNAL TRANSCRIBED SPACER OF NUCLEAR RIBOSOMAL DNA

机译：核糖体DNA内转录间隔中基于子囊菌的核苷酸序列的亲缘关系

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摘要

Nucleotide sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (including the 5.8S ribosomal RNA gene and the flanking ITS1 and ITS2 regions) were used as characters in a phylogenetic analysis of species of Sarcoscypha. The sequences of the ITS regions for 25 isolates, representing fourteen (nine described and five putative new) species of Sarcoscypha, and three outgroup species, Pithya vulgaris, P. cupressina, and Phillipsia domingensis, were determined in both directions using primers ITS1, ITS2, ITS3, and ITS4. The complete sequences ranged from 485 to 764 base pairs in length; they were aligned using the program MALIGN. The sequence of Phillipsia domingensis was highly divergent from that of the other taxa; the alignment was therefore repeated excluding that taxon and subsequent phylogenetic analyses included only the species of Sarcoscypha and one outgroup, Pithya cupressina. The complete alignment contained 907 base pair sites; 446 of these were considered ambiguously aligned and were excluded from phylogenetic analyses. Of the remaining 461 base pair sites, 91 were variable with 43 potentially informative characters. Cladistic analysis yielded two equally parsimonious cladograms with NONA (these corresponded to two fully supported trees of the eight trees found by Hennig86). In all most parsimonious trees, two Asian species, S. striatispora and S. vassiljevae, fell outside of a core group of twelve species which were grouped in two major clades, each including taxa from North America, Europe and Asia. One clade included the following species: S. austriaca, S. coccinea, S. macaronesica, and two putative new species. This clade was divided into two subclades: one consisted of S. macaronesica and S. coccinea, while, in the second, one putative new species from Japan (sp. A) was sister to a polytomy including the putative new species from Taiwan (sp. B) and the S. austriaca isolates from Europe and North America. The other major clade included S. dudleyi and S. emarginata, whose positions varied among the equally parsimonious trees, and a subclade in which the second new species from Japan (sp. D) was sister to a polytomy of S. occidentalis, an isolate of S. javensis, and two putative new species (sp. E from China and sp. C from Ha-wail). In all cases where more than one isolate of the same species was included, those isolates grouped together within each clade, although relationships among the three isolates of S. austriaca and the one of S. sp. B were unresolved. A new combination is made for Peziza emarginata. [References: 56]
机译：核糖核体DNA的内部转录间隔区（ITS）区域（包括5.8S核糖体RNA基因以及侧翼的ITS1和ITS2区域）的核苷酸序列被用作对石棺物种进行系统发育分析的特征。使用引物ITS1，ITS2在两个方向上确定了代表14个（九个描述的和五个推定的新物种）石棺和25个外群物种的Pithya vulgaris，P.cupressina和Phillipsia domingensis的25个分离株的ITS区序列。 ，ITS3和ITS4。完整序列的长度在485至764个碱基对之间。使用程序MALIGN对它们进行对齐。 Phillipis domingensis的序列与其他类群的序列高度不同。因此，重复了该比对，但不包括分类群，随后的系统发育分析仅包括石棺属和一组外来物种，Pithya cupressina。完全比对包含907个碱基对位点;其中446个被认为是歧义对齐的，并从系统发育分析中排除。在其余的461个碱基对位点中，有91个是可变的，具有43个可能提供信息的字符。尾部分析产生了两个具有NONA的同等简约枝形图（这些对应于Hennig86发现的八棵树中的两棵完全支持的树）。在所有最简约的树木中，有两个亚洲树种：S。striatispora和S. vassiljevae，落在由十二个树种组成的核心组之外，这些树种被分为两个主要进化枝，每个进化枝都来自北美，欧洲和亚洲。一个进化枝包括以下物种：南极链球菌，球藻链球菌，玛卡罗尼西亚链球菌和两个假定的新物种。该进化枝分为两个小分支：一个由马氏沙门氏菌和球藻链球菌组成，而在第二个分支中，一个来自日本的推定新物种（sp。A）与包括台湾的推定新物种（sp。 B）和来自欧洲和北美的南方葡萄球菌。其他主要进化枝包括S. dudleyi和S. emarginata，它们的位置在同等的简约树木之间变化，还有一个子巢，其中来自日本的第二个新物种（sp。D）是S. occidentalis的多形体的姐妹，后者是一个分离株。沙门氏菌（S. javensis）和两个推定的新物种（来自中国的E种和来自Ha-wail的C.种）。在所有包括同一物种的一个以上分离株的所有情况下，这些分离株在每个进化枝内分组在一起，尽管三个分离的S. austriaca和S. sp。之间的关系。 B未解决。 Peziza emarginata有了新的组合。 [参考：56]

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