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首页> 外文期刊>Molecular reproduction and development >Detection of PACH1, a nuclear factor implicated in the transcriptionalregulation of meiotic and early haploid stages of spermatogenesis
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Detection of PACH1, a nuclear factor implicated in the transcriptionalregulation of meiotic and early haploid stages of spermatogenesis

机译：检测PACH1，这是一个与精子发生减数分裂和单倍体早期转录调控有关的核因子

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Spermatogenesis occurs in a series of well-defined stages and serves as an excellent model for lineage-specific cell development. Yet, little is known regarding the transcriptional mechanisms responsible for cell- and stage-dependent gene regulation in the male germ line. The rat and mouse proenkephalin genes are expressed from an alternative, spermatogenic cell-specific promoter specifically in meiotically-active pachytene spermatocytes and early post-meiotic spermatids. This promoter thus serves as an excellent model for defining transcriptional regulators involved in germ line-specific gene expression in meiotic cells. Previous transgenic studies identified a proximal, 51 bp 5'-flanking sequence containing two direct repeat elements that are absolutely required for in vivo proenkephalin promoter activity in spermatocytes and spermatids. Here, footprinting analyses were used to further delineate the specific interactions of a spermatogenic cell nuclear factor with the repeat elements within the proximal promoter region. This repeat-binding factor was also shown to be developmentally upregulated specifically in pachytene spermatocytes. Using Southwestern analysis, we have identified a:unique nuclear protein enriched in pachytene spermatocytes that specifically recognizes the repeat elements within the proximal 5'-flanking sequence. We propose that this DNA binding factor, termed PACH 1, is a key transcriptional regulator of the proenkephalin and potentially other gene promoters, uniquely expressed during meiosis in the male germ line. Mel. Reprod. Dev. 57:224-231, 2000.
机译：精子发生发生在一系列明确的阶段，并作为谱系特异性细胞发育的优秀模型。然而，对于负责男性种系中细胞和阶段依赖性基因调控的转录机制知之甚少。大鼠和小鼠前脑啡肽基因是由另一种生精细胞特异性启动子表达的，特别是在减数分裂活跃的粗线精子细胞和减数分裂后早期的精子细胞中表达。因此，该启动子是定义参与减数分裂细胞种系特异性基因表达的转录调控因子的出色模型。先前的转基因研究确定了一个近端51 bp 5'侧翼序列，该序列包含两个直接重复元件，这是精原细胞和精子体内活体前脑啡肽启动子活性绝对必需的。在这里，足迹分析被用来进一步描述生精细胞核因子与近端启动子区域内重复元件的特异性相互作用。还显示该重复结合因子在粗线期精子细胞中发育上调。使用西南分析，我们已经确定了：富含粗线粒细胞精子细胞的独特核蛋白，可以特异性识别近端5'侧翼序列中的重复元件。我们建议，这种称为PACH 1的DNA结合因子是原脑啡肽和其他潜在基因启动子的关键转录调节因子，在雄性生殖系减数分裂过程中独特表达。梅尔责备。开发人员57：224-231，2000。

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