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首页> 外文期刊>Mycologia >Genomic stability of two individuals of Armillaria gallica
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Genomic stability of two individuals of Armillaria gallica

机译：蜜环菌两个个体的基因组稳定性

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Large genetic individuals, or genets, of Armillaria gallica grow mitotically from a single point of origin to occupy discrete territories that often cover several hectares of forest floor. Since the expansion of these individuals must require a large number of cell divisions, somatic mutations are expected. To test this possibility, 22 isolates of A. gallica were collected from throughout the spatial territory of two individuals and 41 anonymous regions of DNA were screened for variation apparent as single strand conformational polymorphism (SSCP). Although there was abundant heterozygosity within each individual and polymorphism between the two individuals, no variation was detected among isolates within either individual. This lack of variation can be explained in two ways: (i) the mutation rate in A. gallica is substantially lower than that of other DNA based microorganisms, and/or (ii) the expansion of A. gallica individuals requires far fewer cell generations than previously estimated. [References: 20]
机译：蜜环菌的大型遗传个体或种系从单个起源点有丝分裂地生长，占据了通常覆盖数公顷森林地面的离散领土。由于这些个体的扩增必须需要大量的细胞分裂，因此预期会有体细胞突变。为了测试这种可能性，从两个人的整个空间区域中收集了22株没食子链霉菌，并筛选了41个DNA匿名区域的变异，表现为单链构象多态性（SSCP）。尽管每个个体内都有丰富的杂合性，并且两个个体之间具有多态性，但在两个个体内的分离株之间均未发现变异。这种变异的缺乏可以用两种方式解释：（i）鸡曲霉的突变率大大低于其他基于DNA的微生物的突变率，和/或（ii）鸡曲霉个体的扩增所需的细胞数少得多比以前估计的要多。 [参考：20]

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