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首页> 外文期刊>Molecular cell >Bacteriophage T4 initiates bidirectional DNA replication through a two-step process.
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Bacteriophage T4 initiates bidirectional DNA replication through a two-step process.

机译:噬菌体T4通过两步过程启动双向DNA复制。

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Two-dimensional gel analysis of the bacteriophage T4 ori(uvsY) region revealed a novel "comet" on the Y arc. This comet contains simple Y molecules in which the branch points map to the ori(uvsY) transcript region. The comet depends on the the origin and DNA synthesis and is abolished by a mutation that reduces replication without affecting transcription. These results argue that the branched molecules are intermediates in replication initiation. A transcriptional terminator, cloned just downstream of the origin promoter, shortened the tail of the comet. Therefore, the location of the transcript determines the DNA branch points. We conclude that the comet DNA consists of intermediates in which unidirectional replication has been triggered by priming from the RNA of the origin R loop.
机译:噬菌体T4 ori(uvsY)区的二维凝胶分析揭示了Y弧上一个新的“彗星”。该彗星包含简单的Y分子,其中的分支点映射到ori(uvsY)转录本区域。彗星取决于起源和DNA合成,并通过减少复制而不影响转录的突变而被废除。这些结果表明,支链分子是复制起始的中间产物。克隆在起源启动子下游的转录终止子缩短了彗星的尾巴。因此,转录物的位置决定了DNA分支点。我们得出的结论是,彗星DNA由中间体组成,其中中间体通过起源R环的RNA引发引发了单向复制。

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