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首页> 外文期刊>Mycologia >Purification of a new isoform of laccase from a Marasmius quercophilus strain isolated from a cork oak litter (Quercus suber L.)
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Purification of a new isoform of laccase from a Marasmius quercophilus strain isolated from a cork oak litter (Quercus suber L.)

机译：从软木栎凋落物（Quercus suber L.）分离的Marasmius quercophilus菌株中纯化新的漆酶同工型。

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摘要

A new isoform of laccase from Marasmius quercophilus is described in this study. The strain of this white-rot fungus was isolated for the first time on a cork oak litter. This isoform exhibited certain common properties of laccases (a molecular weight of 65 Kda, an optimum pH of 6.2 with syringaldazine). But this laccase has also particularly novel features: the best activity measured was observed at high temperatures (80 C) and this isoform was not inhibited with EDTA. Furthermore, this induced laccase was able to transform most of the aromatic compounds tested without the addition of mediators to the reaction mixture, and the transformation of certain chlorophenols (2-chlorophenol and 2,4-dichlorophenol) by a laccase isoform from M. quercophilus is reported here for the first time. We also demonstrate the importance of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) as a mediator since it allowed veratryl alcohol and p-hydroxybenzoic acid transformation. Moreover, new products of transformation were observed using the combination of ABTS with this isoform of laccase. [References: 21]
机译：在这项研究中描述了一种新的来自Marasmius quercophilus的漆酶同工型。首次在软木橡树垃圾上分离出这种白腐真菌菌株。这种同工型表现出漆酶的某些共同特性（分子量为65 Kda，丁香醛嗪的最佳pH为6.2）。但是这种漆酶还具有特别新颖的功能：在高温（80℃）下观察到最佳的活性，而这种同工型并未被EDTA抑制。此外，这种诱导的漆酶能够在不向反应混合物中添加介体的情况下转化大多数测试的芳香族化合物，并且通过来自嗜热毁丝霉菌的漆酶同工型转化某些氯酚（2-氯酚和2,4-二氯酚）。第一次在这里报告。我们还证明了2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS）作为介体的重要性，因为它允许藜芦醇和对羟基苯甲酸转化。此外，结合使用ABTS和这种漆酶同工型，可以观察到转化的新产物。 [参考：21]

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