...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular reproduction and development >The Novel, Actin-Like Protein Tact3 is Expressed in Rodent Testicular Haploid Germ Cells
【24h】

The Novel, Actin-Like Protein Tact3 is Expressed in Rodent Testicular Haploid Germ Cells

机译：新型的肌动蛋白样蛋白Tact3在啮齿动物的睾丸单倍体生殖细胞中表达。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mouse testis actin-like proteins 1 and 2 (mTactl and mTact2), which are expressed in murine haploid germ cells, have been described previously. Here, we report the cloning and characterization of a third actin-like protein from rat, rat testis actin-like protein 3 (rTact3). The complete cDNA of the rTact3 gene was approximately 3.7 kb in length, and its corresponding amino acid sequence consisted of 1219 amino acids. The rTact3 gene lacks introns, similar to mTactl and mTact2. The 356 C-terminal amino acids of rTact3 showed 43% homology with mTactl, whereas the 863 N-terminal amino acids did not show any significant homology. Northern blot analysis revealed that rTact3 mRNA was expressed only in adult rat testes and not during the prepubescent stage. In situ hybridization revealed that rTact3 was expressed exclusively during round and elongated spermatids maturation stages in rat testes. Immunohistochemical experiments using antibodies raised against a synthetic peptide showed that the expression of the rTact3 protein was also restricted in round and elongated spermatids, specifically in the head and acrosome of mature rat sperm. The 5'-flanking region of the mTact3 gene was found to contain a TATA-box motif as well as two putative CREB/c-Jun andfive CI EBP motifs. mTact3 promoter activity was enhanced in a dose-dependent manner by the transfection of CREB, c-Jun, or CIEBP in NIH3T3 cells. These results suggest that Tact3 proteins might play an important role in rodent germ-cell development.
机译：先前已描述了在小鼠单倍体生殖细胞中表达的小鼠睾丸肌动蛋白样蛋白1和2（mTact1和mTact2）。在这里，我们报告从大鼠，大鼠睾丸肌动蛋白样蛋白3（rTact3）的第三个肌动蛋白样蛋白的克隆和表征。 rTact3基因的完整cDNA长度约为3.7 kb，其相应的氨基酸序列由1219个氨基酸组成。 rTact3基因缺少内含子，类似于mTact1和mTact2。 rTact3的356个C末端氨基酸与mTactl具有43％的同源性，而863个N末端氨基酸则无任何显着的同源性。 Northern印迹分析表明，rTact3 mRNA仅在成年大鼠睾丸中表达，而在青春期前不表达。原位杂交表明，rTact3仅在大鼠睾丸的圆形和细长精子成熟阶段表达。使用针对合成肽的抗体进行的免疫组织化学实验表明，rTact3蛋白的表达在圆形和细长的精子中也受到限制，特别是在成熟大鼠精子的头部和顶体中。发现mTact3基因的5'侧翼区域包含一个TATA-box基序以及两个推定的CREB ​​/ c-Jun和五个CI EBP基序。通过在NIH3T3细胞中转染CREB，c-Jun或CIEBP，mTact3启动子活性以剂量依赖性方式增强。这些结果表明，Tact3蛋白可能在啮齿动物生殖细胞发育中起重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号