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首页> 外文期刊>Korean Journal of Horticultural Science & Technology >Effects of extraction condition on extraction efficiency of rubiadin in adventitious roots of noni (Morinda citrifolia).
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Effects of extraction condition on extraction efficiency of rubiadin in adventitious roots of noni (Morinda citrifolia).

机译：提取条件对茜草素不定根中茜草素提取效率的影响。

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摘要

Rubiadin, a major compound of noni (Morinda citrifolia) adventitious root, is highly valued in pharmaceutical industry due to hepatoprotective activity. To dissect rubiadin's effective extraction condition, extraction process of noni adventitious roots was performed with different solvent types, ratio of water to methanol (water, 20, 40, 60, 80, and 100% of methanol), extraction time, and extraction method. In contrast, we also developed a reverse-phase HPLC assay method to determine rubiadin from noni adventitious roots. The HPLC assay of rubiadin was performed by C-18 column using a gradient solvent system of methanol and water with UV detector at 280 nm. The extraction efficiency of different types of solvents were increased in order of methanol (0.08%) > ethanol (0.05%) > acetonitrile (0.03%) > acetone (0.02%) and methylene chloride (0.02%). The results of rubiadin extraction using different solvents showed that 1 hour of ultrasonic extraction was effective in order of 60% methanol (0.21%) > 80% methanol (0.13%) > 100% methanol (0.07%), 40% methanol (0.07%) and 2 hours of reflux extraction was effective in order of 60% methanol (0.21%) > 40% methanol (0.17%) > 80% methanol (0.14%). To compare the extraction efficiency of rubiadin according to the extraction methods and time for high rubiadin content, the extracts of rubiadin in noni adventitious roots were isolated with the methods of ultrasonic extraction, shaking extraction and reflux extraction. Rubiadin extracted from the methods of ultrasonic waves and shaking displayed the highest contents at 8 and 24 hours, respectively.
机译：茜草素是诺丽（Morinda citrifolia ）不定根的主要化合物，由于具有保肝作用，在制药工业中具有很高的价值。为了剖析茜草素的有效提取条件，在不同溶剂类型，水与甲醇的比例（水，甲醇的20％，40％，60％，80％和100％），提取时间和提取方法下进行了不定根的提取过程。相反，我们还开发了反相HPLC分析方法，可从非定居根中测定茜草素。通过C-18色谱柱，使用甲醇和水的梯度溶剂系统以及280 nm的UV检测器，进行茜草素的HPLC分析。按甲醇（0.08％）>乙醇（0.05％）>乙腈（0.03％）>丙酮（0.02％）和二氯甲烷（0.02％）的顺序增加不同类型溶剂的提取效率。使用不同溶剂提取的茜草素的结果表明，超声提取1小时是有效的，依次为60％甲醇（0.21％）> 80％甲醇（0.13％）> 100％甲醇（0.07％），40％甲醇（0.07％） ）和2小时的回流萃取有效，依次为60％甲醇（0.21％）> 40％甲醇（0.17％）> 80％甲醇（0.14％）。为了比较茜草素按提取方法和提取时间对高茜草素含量的提取效率，采用超声提取，摇动提取和回流提取的方法，分离了不定根中茜草素的提取物。从超声波和摇动方法中提取的茜草素分别在8小时和24小时时显示出最高含量。

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