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首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >Antibacterial Effects and Cellular Responses of Imipenem-resistant Pseudomonas aeruginosa Exposed to Green Tea Polyphenols
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Antibacterial Effects and Cellular Responses of Imipenem-resistant Pseudomonas aeruginosa Exposed to Green Tea Polyphenols

机译：绿茶多酚暴露对亚胺培南耐药的铜绿假单胞菌的抗菌作用和细胞响应

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摘要

The aim of this work was to investigate the synergically bactericidal effects and cellular responses of tea polyphenols (TPP) and imipenem on imipenem-resistant Pseudomonas aeruginosa. Imipenem-resistant Ps. aeruginosa was isolated from patient in hospital. The bactericidal effects of TPP and imipenem were evaluated on the basis of its minimum inhibitory concentrations (MIC). The combined use of TPP and imipenem resulted in 16-fold and 8-fold reductions in the MICs of imipenem for the imipenem-susceptible and imipenem-resistant Ps. aeruginosa, respectively. The bactericidal effects of the imipenem and TPP against the Ps. aeruginosa was evaluated using the time-kill assay. The syn-ergetic effects of the combinations of TPP and imipenem against Ps. aeruginosa were confirmed. Western blot using anti-DnaK and anti-GroEL monoclonal antibodies was performed to investigate the expression of stress shock proteins (SSPs) in imipenem-susceptible and imipenem-resistant strains exposed to TPP. The amount of SSPs were induced as the exposure time increased and decreased. The molecular weights of DnaK and GroEL were 70 kDa and 60 kDa, respectively. SDS-PAGE with silver staining revealed that the amount of lipopolysaccharides (LPS) increased or decreased in thestrain treated to different concentrations and exposing periods of TPP. Scanning electron microscopic analysis demonstrated the presence of umblicated and wrinkled surfaces for cells treated with TPP or imipenem.
机译：这项工作的目的是研究茶多酚（TPP）和亚胺培南对耐亚胺培南的铜绿假单胞菌的协同杀菌作用和细胞应答。耐亚胺培南的PS。从医院的患者中分离出铜绿。根据TPP和亚胺培南的最小抑菌浓度（MIC）评估其杀菌效果。 TPP和亚胺培南的组合使用可导致亚胺培南易感和亚胺培南的Ps亚胺培南的MIC降低16倍和8倍。分别为铜绿。亚胺培南和TPP对Ps的杀菌作用。使用时间杀灭试验评估铜绿。 TPP和亚胺培南组合对Ps的协同作用。确认了铜绿。使用抗DnaK和抗GroEL单克隆抗体进行了蛋白质印迹试验，以研究应激冲击蛋白（SSP）在暴露于TPP的亚胺培南敏感和亚胺培南耐药菌株中的表达。随着暴露时间的增加和减少，诱导了SSP的量。 DnaK和GroEL的分子量分别为70 kDa和60 kDa。带有银染的SDS-PAGE表明，在不同浓度和TPP暴露时间下，菌株中的脂多糖（LPS）数量增加或减少。扫描电子显微镜分析表明，用TPP或亚胺培南处理的细胞存在明显的皱纹表面。

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