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Selective probing and imaging of cells with single walled carbon nanotubes as near-infrared fluorescent molecules

机译：具有单壁碳纳米管作为近红外荧光分子的细胞的选择性探测和成像

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摘要

Fluorescent molecules emitting in the near-infrared (NIR, wavelength similar to 0.8-2 mu m) are relatively scarce and have been actively sought for biological applications because cells and tissues exhibit little auto-fluorescence in this region. Here, we report the use of semiconducting single-walled carbon nanotubes (SWNTs) as near-infrared fluorescent tags for selective probing of cell surface receptors and cell imaging. Biologically inert SWNTs with polyethyleneglycol functionalization are conjugated to antibodies such as Rituxan to selectively recognize CD20 cell surface receptor on B-cells with little nonspecific binding to negative T-cells and Herceptin to recognize HER2eu positive breast cancer cells. We image selective SWNT-antibody binding to cells by detecting the intrinsic NIR photoluminescence of nanotubes. We observe ultralow NIR autofluorescence for various cells, an advantageous feature over high autofluorescence and large variations between cells lines in the visible, This establishes SWNTs as novel NIR fluorophors for sensitive and selective biological detections and imaging in vitro and potentially in vivo. Further, our results clearly show that the interactions between carbon nanotubes and living cells are strongly dependent on surface functionalization of nanotubes.
机译：由于细胞和组织在该区域几乎没有自发荧光，因此在近红外（NIR，类似于0.8-2μm的波长）中发射的荧光分子相对稀缺，并已被积极地用于生物学应用。在这里，我们报告使用半导体单壁碳纳米管（SWNTs）作为近红外荧光标签，用于选择性探测细胞表面受体和细胞成像。将具有聚乙二醇功能的生物惰性SWNT与抗体（如利妥昔单抗）偶联，以选择性识别B细胞上的CD20细胞表面受体，而几乎没有与阴性T细胞的非特异性结合，而赫赛汀则可以识别HER2 / neu阳性乳腺癌细胞。我们通过检测纳米管的内在近红外光致发光成像选择性SWNT抗体结合细胞。我们观察到各种细胞的超低NIR自发荧光，这是优于高自发荧光的有利特征，并且可见细胞系之间存在较大差异。这将SWNTs确立为新型NIR荧光体，用于体外和潜在体内的敏感和选择性生物学检测和成像。此外，我们的结果清楚地表明，碳纳米管与活细胞之间的相互作用强烈依赖于纳米管的表面功能化。

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