• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Microbial Biotechnology >Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters.
【24h】

Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters.

机译：工程链霉菌变色链霉菌用于次级代谢产物基因簇的异源表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have constructed derivatives of Streptomyces coelicolor M145 as hosts for the heterologous expression of secondary metabolite gene clusters. To remove potentially competitive sinks of carbon and nitrogen, and to provide a host devoid of antibiotic activity, we deleted four endogenous secondary metabolite gene clusters from S. coelicolor M145--those for actinorhodin, prodiginine, CPK and CDA biosynthesis. We then introduced point mutations into rpoB and rpsL to pleiotropically increase the level of secondary metabolite production. Introduction of the native actinorhodin gene cluster and of gene clusters for the heterologous production of chloramphenicol and congocidine revealed dramatic increases in antibiotic production compared with the parental strain. In addition to lacking antibacterial activity, the engineered strains possess relatively simple extracellular metabolite profiles. When combined with liquid chromatography and mass spectrometry, we believe that these genetically engineered strains will markedly facilitate the discovery of new compounds by heterologous expression of cloned gene clusters, particularly the numerous cryptic secondary metabolic gene clusters that are prevalent within actinomycete genome sequences. 2010 The Authors. Journal compilation 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.Registry Number/Name of Substance 0 (Anti-Bacterial Agents). 0 (Bacterial Proteins).
机译：我们已经构建了Streptomyces coelicolor M145的衍生物作为宿主，用于次级代谢产物基因簇的异源表达。为了去除潜在的竞争性碳和氮汇，并提供没有抗生素活性的宿主，我们从天蓝色链霉菌M145中删除了四个内源性次级代谢产物基因簇-分别用于放线菌丝蛋白，脯氨酸，CPK和CDA的生物合成。然后，我们将点突变引入rpoB和rpsL中，以多效性增加次级代谢产物的产生水平。引入天然的放线菌丝蛋白基因簇和用于氯霉素和刚果金的异源生产的基因簇表明，与亲本菌株相比，抗生素的生产显着增加。除了缺乏抗菌活性外，工程菌株还具有相对简单的细胞外代谢物谱。当与液相色谱和质谱联用时，我们相信这些基因工程菌株将通过异源表达克隆的基因簇，特别是放线菌基因组序列中普遍存在的众多隐性次级代谢基因簇，显着促进新化合物的发现。 2010作者。期刊汇编，2010年应用微生物学协会和Blackwell Publishing Ltd.注册编号/物质名称0（抗细菌剂）。 0（细菌蛋白）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号