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Functional gene silencing mediated by chitosan/siRNA nanocomplexes

机译：壳聚糖/ siRNA纳米复合物介导的功能基因沉默

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摘要

Chitosan/siRNA nanoparticles to knock down FHL2 gene expression were reported in this work. The physicochemical properties such as particle size, surface charge, morphology and complex stability of chitosan nanoparticle-incorporated siRNA were evaluated. Nanoparticles which were formulated with chitosan/siRNA exhibited irregular, lamellar and dendritic structures with a hydrodynamic radius size of about 148 nm and net positive charges with zeta-potential value of 58.5 mV. The knockdown effect of the chitosan/siRNA nanoparticles on gene expression in FHL2 over-expressed human colorectal cancer Lovo cells was investigated.The result showed that FHL2 siRNA formulated within chitosan nanoparticles could knock down about 69.6% FHL2 gene expression, which is very similar to the 68.8% reduced gene expression when siRNA was transfected with liposome Lipofectamine. Western analysis further showed significant FHL-2 protein expression reduced by the chitosan/siRNA nanoparticles. The results also showed that blocking FHL2 expression by siRNA could also inhibit the growth and proliferation of human colorectal cancer Lovo cells. The current results demonstrated that chitosan-based siRNA nanoparticles were a very efficient delivery system for siRNA in vivo as previously reported.
机译：在这项工作中报道了壳聚糖/ siRNA纳米颗粒可降低FHL2基因的表达。评价了壳聚糖纳米粒子掺入的siRNA的理化性质，如粒径，表面电荷，形态和复合物稳定性。用壳聚糖/ siRNA配制的纳米颗粒表现出不规则，层状和树状结构，其流体力学半径尺寸约为148 nm，净正电荷的ζ电位值为58.5 mV。研究了壳聚糖/ siRNA纳米粒对FHL2过表达的人结直肠癌Lovo细胞基因表达的抑制作用，结果表明，壳聚糖纳米粒中配制的FHL2 siRNA可以抑制约69.6％的FHL2基因表达，与用脂质体脂质体脂质体转染siRNA后，基因表达降低了68.8％。 Western分析进一步表明，壳聚糖/ siRNA纳米颗粒可显着降低FHL-2蛋白的表达。结果还表明，通过siRNA阻止FHL2表达还可以抑制人大肠癌Lovo细胞的生长和增殖。当前的结果表明，基于壳聚糖的siRNA纳米颗粒是体内有效的siRNA递送系统，如先前报道的那样。

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