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HYDROLYSIS OF DIHYDROURIDINE AND RELATED COMPOUNDS

机译：双氢尿嘧啶及其相关化合物的水解

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摘要

Dihydrouridine is absent from the tRNA of almost all hyperthermophiles and most Archaea but is ubiquitous in the tRNA of Eubacteria and Eukaryotes. In order to investigate whether this could be due to instability, the rate of ring opening of dihydrouridine was measured between 25 and 120 degrees C. The dihydrouridine ring is stable at 25 degrees C, but the half-life at 100 degrees C and pH 7 is 9.1 h, which is comparable to the doubling time of hyperthermophiles. This suggests an explanation for the absence of dihydrouridine from the tRNA of hyperthermophiles. The rates of ring opening of dihydrouracil, dihydrothymine, and 1-N-methyldihydrouracil were measured at 100 degrees C and pH 6-9, as were the equilibrium constants for ring closure of the ureido acids to the dihydrouracils. The pH rate profiles for ring opening and ring closing were calculated from the data. Possible roles for dihydrouracils in the pre-RNA world are discussed.
机译：几乎所有嗜高温菌和大多数古生菌的tRNA中都不存在二氢尿苷，但在真细菌和真核生物的tRNA中普遍存在二氢尿苷。为了研究这是否可能是由于不稳定性造成的，测量了二氢尿苷的开环速率在25至120摄氏度之间。二氢尿苷环在25摄氏度下稳定，但在100摄氏度和pH 7下的半衰期是9.1小时，相当于超嗜热菌的倍增时间。这表明对超嗜热菌的tRNA中不存在二氢尿苷的解释。在100℃和pH 6-9下测量二氢尿嘧啶，二氢胸腺嘧啶和1-N-甲基二氢尿嘧啶的开环速率，以及脲基酸与二氢尿嘧啶的闭环平衡常数。由数据计算开环和闭环的pH速率曲线。讨论了二氢尿嘧啶在前RNA世界中的可能作用。

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