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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >The source of brain adenosine outflow during ischemia and electrical stimulation.
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The source of brain adenosine outflow during ischemia and electrical stimulation.

机译：缺血和电刺激过程中脑腺苷外流的来源。

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摘要

Adenosine outflow and adenosine and adenine nucleotide content of hippocampal slices were evaluated under two different experimental conditions: ischemia-like conditions and electrical stimulation (10 Hz). Five minutes of ischemia-like conditions brought about an 8-fold increase in adenosine outflow in the following 5 min during reperfusion, and a 2-fold increase in adenosine content, a 43% decrease in ATP, a 72% increase in AMP and a 30% decrease in energy charge (E.C.) at the end of the ischemic period. After 10 min of reperfusion ATP, AMP and E.C. returned to control values, while the adenosine content was further increased. Five minutes of electrical stimulation brought about an 8-fold increase in adenosine outflow that peaked 5 min after the end of stimulation, a 4-fold increase in adenosine content and an 18% decrease in tissue E.C. at the end of stimulation. After 10 min of rest conditions the adenosine content and E.C. returned to basal values. The origin of extracellular adenosine from S-adenosylhomocysteine (SAH) was examined under the two different experimental conditions. The SAH hydrolase inhibitor, adenosine-2,3-dialdehyde (10 microM), does not significantly modify the adenosine outflow evoked by electrical stimulation or ischemia-like conditions. This finding excludes a significant contribution by the transmethylation pathway to adenosine extracellular accumulation evoked by an electrical or ischemic stimulus, and confirms that the most likely source of adenosine is from AMP dephosphorylation.
机译：在两种不同的实验条件下评估海马切片的腺苷流出量以及腺苷和腺嘌呤核苷酸含量：缺血样条件和电刺激（10 Hz）。五分钟的缺血样情况在再灌注后的5分钟内使腺苷流出增加了8倍，腺苷含量增加了2倍，ATP减少了43％，AMP减少了72％，在缺血期结束时，能量电荷（EC）降低30％。再灌注10分钟后，ATP，AMP和E.C.恢复到对照值，而腺苷含量进一步增加。五分钟的电刺激使腺苷流出增加了8倍，在刺激结束后5分钟达到峰值，在刺激结束时腺苷含量增加了4倍，组织E.C.降低了18％。休息10分钟后，腺苷含量和E.C.恢复到基础值。在两个不同的实验条件下检查了来自S-腺苷同型半胱氨酸（SAH）的细胞外腺苷的来源。 SAH水解酶抑制剂，腺苷2,3-二醛（10 microM），不会显着改变电刺激或类似缺血的情况引起的腺苷流出。这一发现排除了转甲基化途径对由电刺激或缺血刺激引起的腺苷细胞外积累的重大贡献，并证实腺苷最可能的来源是AMP脱磷酸作用。

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