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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Expressions of amyloid precursor protein, synaptophysin and presenilin-1 in the different areas of the developing cerebellum of rat.
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Expressions of amyloid precursor protein, synaptophysin and presenilin-1 in the different areas of the developing cerebellum of rat.

机译：淀粉样前体蛋白，突触素和早老素-1在大鼠发育中小脑的不同区域中的表达。

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This study reveals the expressions of Alzheimer's disease-related amyloid precursor protein, presenilin-1, and a presynaptic marker protein, synaptophysin, in the archi-, paleo- and neocerebellum during the postnatal development of the rat. The Western blot results demonstrate a gradual increase in the soluble amyloid precursor protein level in the archicerebellum during the first 3 weeks, while in the neo- and paleocerebellum the levels reach a plateau as early as the 1st week. Immunohistochemically, the protein is present in the deep part of the external granule cell layer and the internal granule cell layer in the newborn animal, while in 3-week-old animals the staining appears mainly in the perikarya and dendrites of the Purkinje cells. The level of synaptophysin increases progressively from postnatal day 7 up to 3 weeks in the archi- and paleocerebellum, and up to 6 weeks in the neocerebellum. Immunohistochemically, the amyloid precursor protein staining appears first in the inner part of the molecular layer and in the internal granule cell layer. In a 3-week-old animal, synaptophysin staining is present in all areas of the cerebellar molecular layer and in the internal granule cell layer. The presenilin-1 immunohistochemical reaction appeared equally in the archi-, paleo- and neocerebellum. Much of the staining is present in the glial cells and Purkinje cells. Less immunoreactivity is observed in the Golgi cells and granule cells. It is concluded that the postnatal expressions of soluble and membrane-bound amyloid precursor protein, synaptophysin and presenilin-1 are regulated differently during the ontogenetical development of the archi-, paleo- and neocerebellum of rat. Further, the amyloid precursor protein and presenilin-1 may be present in cells which do not degenerate in Alzheimer's disease.
机译：这项研究揭示了阿尔茨海默氏病相关淀粉样蛋白前体蛋白presenilin-1和突触前标记蛋白突触素在大鼠新生后足the，古脑和新小脑中的表达。 Western印迹结果表明，在前3周中，小脑弓形虫中可溶性淀粉样前体蛋白水平逐渐增加，而在新小脑和小脑中，该水平早在第1周就达到了平稳状态。免疫组织化学分析，该蛋白质存在于新生动物的外部颗粒细胞层和内部颗粒细胞层的深部，而在3周龄的动物中，染色主要出现在Purkinje细胞的周核和树突中。突触素的水平从出生后第7天开始逐渐增加，在小脑和小脑中达到3周，而在新小脑中则达到6周。在免疫组织化学上，淀粉样前体蛋白染色首先出现在分子层的内部和内部颗粒细胞层。在三周大的动物中，小脑分子层的所有区域和内部颗粒细胞层都存在突触素染色。 presenilin-1免疫组织化学反应在原发，古发和新小脑中均出现。大部分染色存在于神经胶质细胞和浦肯野细胞中。在高尔基体细胞和颗粒细胞中观察到较少的免疫反应性。结论是，在大鼠前小脑，古小脑和新小脑的个体发育过程中，可溶性和膜结合的淀粉样前体蛋白，突触素和早老素-1的产后表达受到不同的调节。此外，淀粉样蛋白前体蛋白和早老素-1可以存在于在阿尔茨海默氏病中不退化的细胞中。

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