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首页> 外文期刊>Neuron glia biology >Influence of adult Schwann cells and olfactory ensheathing glia on axontarget cell interactions in the CNS a comparative analysis using a retinotectal cograft model
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Influence of adult Schwann cells and olfactory ensheathing glia on axontarget cell interactions in the CNS a comparative analysis using a retinotectal cograft model

机译：成年雪旺细胞和嗅鞘神经胶质细胞对中枢神经系统轴突靶细胞相互作用的影响

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摘要

We used an in vivo transplant approach to examine how adult Schwann cells and olfactory ensheathing glia OEG influence the specificity of axontarget cell interactions when they are introduced into the CNS. Populations of either Schwann cells or OEG were mixed with dissociated fetal tectal cells presumptive superior colliculus and, after reaggregation, pieces were grafted onto newborn rat superior colliculus. Both glial types were prelabeled with lentiviral vectors encoding green fluorescent protein. Grafts rapidly established fiber connections with the host and retinal projections into cografts were assessed 656 days posttransplantation by injecting cholera toxinB into host eyes. In control rats that received pure dissociatedreaggregated tectal grafts, retinal ganglion cell RGC axons selectively innervated defined target areas, corresponding to the retinorecipient layer in normal superior colliculus. The pattern of RGC axon ingrowth into OEG containing cografts was similar to that in control grafts. However, in Schwann cell cografts there was reduced host retinal input into presumptive target areas and many RGC axons were scattered throughout the neuropil. Given that OEG in cografts had minimal impact on axontarget cell recognition, OEG might be an appropriate cell type for direct transplantation into injured neuropil when attempting to stimulate specific pathway reconstruction.
机译：我们使用了一种体内移植方法来检查将成年的雪旺氏细胞和嗅鞘神经胶质细胞OEG在引入中枢神经系统后如何影响轴突靶细胞相互作用的特异性。将雪旺氏细胞或OEG的细胞群与分离的胎儿上皮细胞推定的上丘混合在一起，重新聚集后，将其移植到新生大鼠上丘。两种神经胶质类型均用编码绿色荧光蛋白的慢病毒载体预先标记。移植后656天，通过将霍乱毒素B注入宿主眼中，评估了与宿主之间快速建立的纤维连接以及与共植体的视网膜投影。在接受纯离体再聚集的顶盖移植物的对照大鼠中，视网膜神经节细胞RGC轴突选择性地支配了定义的靶区域，该区域对应于正常上丘的视网膜受体层。 RGC轴突向内生长到包含OEG的同种异体移植物中的模式与对照移植物中的相似。然而，在施万细胞移植中，减少了宿主向假定靶区域的视网膜输入，并且许多RGC轴突散布在整个神经桩中。鉴于同种移植物中的OEG对轴突靶细胞识别的影响极小，因此当尝试刺激特异性途径重建时，OEG可能是直接移植到受损神经纤维中的合适细胞类型。

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