...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neuroendocrinology: International Journal for Basic and Clinical Studies on Neuroendocrine Relationships >17 beta-Hydroxysteroid dehydrogenase activity correlates with the type-2 17 beta-hydroxysteroid dehydrogenase mRNA abundance in human meningioma tumors.
【24h】

17 beta-Hydroxysteroid dehydrogenase activity correlates with the type-2 17 beta-hydroxysteroid dehydrogenase mRNA abundance in human meningioma tumors.

机译：人类脑膜瘤肿瘤中的17β-羟基类固醇脱氢酶活性与2型17β-羟基类固醇脱氢酶mRNA丰度相关。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Benign meningioma tumors possess significant levels of 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) activity. Two different 17 beta-HSDs were discovered in human placenta: one highly estrogen specific and using NADP+/NADPH as cofactors (type-1 17 beta-HSD), and a second one that utilizes both androgens and estrogens as substrates with NAD+/NADH (type-2 17 beta-HSD). Recently, two further human 17 beta-HSDs were isolated. A testis-specific 17 beta-HSD (type-3 17 beta-HSD) favors the reduction of delta 4-androstenedione to testosterone, and a ubiquitously expressed type-4 17 beta-HSD preferentially catalyzes the oxidation of estradiol and delta 5-androstenediol. In this study we characterize the expression levels of different types of 17 beta-HSD in a wide series of tumors. Using the Northern blotting method we show that type-1, -3, and -4 17 beta-HSDs are not detectable in meningiomas. In contrast, the type-2 17 beta-HSD RNA is present in 6 of 17 meningiomas and its abundance is directly correlated with estrogenic 17 beta-HSD activity (r2 = 0.74). The presence of type-2 17 beta-HSD is also demonstrated by in situ hybridization. RT-PCR and Western blots show that type-4 17 beta-HSD is also present, though at much lower levels. The progesterone receptor level, the epidermal growth factor receptor level, and the age of the patients are not correlated with the estrogenic 17 beta-HSD activity or type-2 17 beta-HSD mRNA expression level.
机译：良性脑膜瘤肿瘤具有显着水平的17β-羟类固醇脱氢酶（17β-HSD）活性。在人胎盘中发现了两种不同的17β-HSD：一种是高度雌激素特异性的，使用NADP + / NADPH作为辅因子（1型17β-HSD），另一种利用雄激素和雌激素作为NAD + / NADH的底物（ 2型17 beta-HSD）。最近，又分离出两个人类17β-HSD。睾丸特异性的17 beta-HSD（3型17 beta-HSD）有助于将δ4-雄烯二酮还原为睾丸激素，而普遍表达的4型17β-HSD则优先催化雌二醇和δ5-雄烯二醇的氧化。 。在这项研究中，我们表征了一系列肿瘤中不同类型的17β-HSD的表达水平。使用Northern印迹方法，我们显示在脑膜瘤中无法检测到类型1、3和-3 17β-HSD。相反，在17个脑膜瘤中有6个存在2型17β-HSDRNA，其丰度与雌激素17β-HSD活性直接相关（r2 = 0.74）。还通过原位杂交证明了2型17β-HSD的存在。 RT-PCR和蛋白质印迹显示，虽然其水平要低得多，但也存在4型17β-HSD。孕激素受体水平，表皮生长因子受体水平和患者年龄与雌激素17β-HSD活性或2型17β-HSDmRNA表达水平无关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号