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首页> 外文期刊>New biotechnology >Highly efficient bioethanol production by a Saccharomyces cerevisiae train with multiple stress tolerance to high temperature, acid and ethanol
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Highly efficient bioethanol production by a Saccharomyces cerevisiae train with multiple stress tolerance to high temperature, acid and ethanol

机译：通过酿酒酵母培养的高效生物乙醇生产，对高温，酸和乙醇具有多重压力耐受性

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Use of super strains exhibiting tolerance to high temperature, acidity and ethanol is a promising way to make ethanol production economically feasible. We describe here the breeding and performance of such a multiple-tolerant strain of Saccharomyces cerevisiae generated by a spore-to-cell hybridization technique without recombinant DNA technology. A heterothallic strain showing a high-temperature (418C) tolerant (Htg~+) phenotype, a derivative from a strain isolated from nature, was crossed with a homothallic strain displaying high-ethanol productivity (Hep~+), a stock culture at the Thailand Institute of Scientific and Technological Research. The resultant hybrid TJ14 displayed ability to rapidly utilize glucose, and produced ethanol (46.6 g/l) from 10% glucose fermentation medium at high temperature (418C). Not only ethanol productivity at 418C but also acid tolerance (Acd~+) was improved in TJ14 as compared with its parental strains, enabling TJ14 to grow in liquid medium even at pH 3. TJ14 maintained high ethanol productivity (46.0 g/l) from 10% glucose when fermentation was done under multiple-stress conditions (418C and pH 3.5). Furthermore, when TJ14 was subjected to a repeated-batch fermentation scheme, the growth and ethanol production of TJ14 were maintained at excellent levels over ten cycles of fermentation. Thus, the multiple-stress (Htg~+ Hep~+ Acd~+) resistant strain TJ14 should be useful for cost-effective bioethanol production under high-temperature and acidic conditions.
机译：使用对高温，酸度和乙醇具有耐受性的超级菌株是使乙醇生产在经济上可行的有希望的方法。我们在这里描述了通过无重组DNA技术的孢子细胞杂交技术产生的啤酒酵母的这种多耐受菌株的选育和性能。将具有高温（41​​8C）耐受性（Htg〜+）表型的杂种菌株（从自然界分离出的菌株衍生）与具有高乙醇生产率（Hep〜+）的同型菌株杂交，该菌株在泰国科学技术研究所。所得的杂种TJ14显示出快速利用葡萄糖的能力，并在高温（41​​8℃）下由10％葡萄糖发酵培养基生产出乙醇（46.6 g / l）。与亲本菌株相比，TJ14不仅提高了418°C的乙醇生产率，而且耐酸性（Acd〜+）都得到了提高，即使在pH 3的条件下，TJ14仍可以在液体培养基中生长。TJ14保持了较高的乙醇生产率（46.0 g / l）在多重压力条件（418C和pH 3.5）下进行发酵时，葡萄糖含量为10％。此外，当将TJ14进行重复分批发酵方案时，在十个发酵周期中，TJ14的生长和乙醇产量保持在优异水平。因此，耐多应力（Htg〜+ Hep〜+ Acd〜+）菌株TJ14可用于在高温和酸性条件下生产具有成本效益的生物乙醇。

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