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首页> 外文期刊>Neuroscience Letters: An International Multidisciplinary Journal Devoted to the Rapid Publication of Basic Research in the Brain Sciences >An adenosine triphosphate (ATP)-sensitive K+ channel of rat neocortical neurons is bi-gated by intracellular ATP and voltage: a novel channel gating mechanism?
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An adenosine triphosphate (ATP)-sensitive K+ channel of rat neocortical neurons is bi-gated by intracellular ATP and voltage: a novel channel gating mechanism?

机译：大鼠新皮层神经元对三磷酸腺苷（ATP）敏感的K +通道被细胞内ATP和电压双向阻断：一种新型的通道门控机制？

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摘要

To determine whether the adenosine triphosphate (ATP)-sensitive K+ (K-ATP) channels, which are suggested to be mainly regulated by intracellular ATP or other kinds of triphosphate nucleotides, are gated by membrane potentials (Vm), single K-ATP channel currents were studied on inside-out membrane patches of neurons acutely dissociated from Sprague-Dawley rat neocortex. The K-ATP channels recorded have a unitary conductance from 96.97 +/- 5.32 pS (n = 11) at potentials of approximately 10-60 mV to 98.31 +/- 3.26 pS (n = 11) at approximately -10 to -60 mV. Besides being inhibited by cytoplasmic ATP, channel kinetics was also affected by Vm. Open- and closed-time histograms were well fitted by 2 exponentials, suggesting that the channels have 2 open and closed states. Mean open time (tau om), open probability increased while mean closed time (tau cm) decreased with depolarization. The fitted equations of the relationships between Vm and those kinetic parameters may be described as: tau om = -159.26lnV + 403.64, Po = -0.01Vm2 + 0.08Vm + 0.87 and tau cm = 0.17Vm3-2.5Vm2 + 10.35Vm - 7.68, respectively. We suggest that the K-ATP channels be bi-gated by both intracellular ATP and membrane potentials. This property of the neuronal K-ATP channels may be related to their pathophysiological functions.
机译：要确定是否主要由细胞内ATP或其他种类的三磷酸核苷酸调节的三磷酸腺苷（ATP）敏感的K +（K-ATP）通道是否被膜电位（Vm）选通，单个K-ATP通道研究了从Sprague-Dawley大鼠新皮层急性分离的神经元的由内而外的膜片电流。记录的K-ATP通道在大约10-60 mV的电位下从96.97 +/- 5.32 pS（n = 11）的单位电导在大约-10到-60 mV的电位下为98.31 +/- 3.26 pS（n = 11）的单位电导。除了受细胞质ATP抑制外，通道动力学还受Vm影响。打开时间和关闭时间直方图通过2个指数很好地拟合，这表明通道具有2个打开状态和关闭状态。平均打开时间（tau om），打开概率随着去极化而增加，而平均关闭时间（tau cm）减少。 Vm与那些动力学参数之间关系的拟合方程可描述为：tau om = -159.26lnV + 403.64，Po = -0.01Vm2 + 0.08Vm + 0.87和tau cm = 0.17Vm3-2.5Vm2 + 10.35Vm-7.68 ， 分别。我们建议，K-ATP通道被细胞内ATP和膜电位同时控制。神经元K-ATP通道的这种特性可能与其病理生理功能有关。

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