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首页> 外文期刊>New Journal of Chemistry >Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins
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Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins

机译：硼酸通过硫醇-烯点击化学功能化的磁性纳米粒子，用于糖蛋白的选择性富集

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摘要

A simple, novel approach was developed for the preparation of boronic acid functionalized Fe3O4 magnetic nanoparticles (MNPs) via thiol-ene (TE) click reaction. In this work, two clickable Fe3O4 MNPs functionalized with either alkene or thiol moieties were synthesized. Firstly, Fe3O4 MNPs were synthesized through a solvothermal method and then the clickable alkene- or thiol-coated Fe3O4 MNPs were prepared by sol-gel reaction with an organosilicon coupling agent, 3-(methacryloyloxy) propyttrimethoxylsilane (MPS) or 3-mercaptopropyltriethoxysilane (MPTES). The carbon-carbon double bonds/thiol groups on the surface of the MNPs serve as clickable sites to react with 4-mercaptophenylboronic acid (4-MPBA)/ 3-acrylamidophenylboronic acid (AAPBA) during the subsequent TE click reaction. Finally, the high density of boronic acid ligands immobilized on the surface of the Fe3O4 MNPs was obtained via TE click reactions. The morphology, adsorption and recognition properties of the Fe3O4 MNPs were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), vibrating sample magrietometry (VSM) and X-ray photoelectron spectrometry (XPS). Four proteins, including ovalbumin (OB) and transferrin (Trf) as glycoprotein templates, and lysozyme (Lyz) and horse heart cytochrome c (Cyt C) as non-glycoprotein templates are chosen as target proteins. Two types of click-Fe3O4 MNP (Fe3O4@MPS@PBA and Fe3O4@SH@AAPBA) exhibit a high binding capacity and excellent specificity towards glycoproteins, and can selectively capture and separate glycoproteins from egg white samples directly. Furthermore, this work could provide a promising method of, surface modification for the design of more efficient adsorbents for the isolation and enrichment of proteins from complex bio-samples.
机译：开发了一种简单新颖的方法，用于通过硫醇-烯（TE）单击反应制备硼酸官能化的Fe3O4磁性纳米颗粒（MNP）。在这项工作中，合成了两个可点击的Fe3O4 MNP，它们被烯烃或硫醇部分官能化。首先，通过溶剂热法合成Fe3O4 MNP，然后与有机硅偶联剂，3-（甲基丙烯酰氧基）丙三甲氧基硅烷（MPS）或3-巯基丙基三乙氧基硅烷（MPTES）通过溶胶-凝胶反应制备可点击的烯或硫醇包覆的Fe3O4 MNP。 ）。 MNP表面上的碳-碳双键/硫醇基团是可点击的位置，可在随后的TE点击反应中与4-巯基苯基硼酸（4-MPBA）/ 3-丙烯酰胺基苯基硼酸（AAPBA）反应。最后，通过TE点击反应获得了固定在Fe3O4 MNP表面上的高密度硼酸配体。通过透射电子显微镜（TEM），扫描电子显微镜（SEM），X射线衍射（XRD），振动样品比色法（VSM）和X射线光电子能谱（XPS）研究了Fe3O4 MNP的形态，吸附和识别特性。 ）。选择四种蛋白，包括卵白蛋白（OB）和转铁蛋白（Trf）作为糖蛋白模板，以及溶菌酶（Lyz）和马心脏细胞色素c（Cyt C）作为非糖蛋白模板作为目标蛋白。两种类型的click-Fe3O4 MNP（Fe3O4 @ MPS @ PBA和Fe3O4 @ SH @ AAPBA）显示出高结合能力和对糖蛋白的优异特异性，并且可以选择性地直接从蛋清样品中捕获和分离糖蛋白。此外，这项工作可以提供一种有前途的表面修饰方法，用于设计更有效的吸附剂，以从复杂的生物样品中分离和富集蛋白质。

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