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首页> 外文期刊>Nucleic Acids Research >THE MURINE IGM SECRETORY POLY(A) SITE CONTAINS DUAL UPSTREAM AND DOWNSTREAM ELEMENTS WHICH AFFECT POLYADENYLATION
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THE MURINE IGM SECRETORY POLY(A) SITE CONTAINS DUAL UPSTREAM AND DOWNSTREAM ELEMENTS WHICH AFFECT POLYADENYLATION

机译：小鼠IGM securetory poly（a）网站包含影响聚丙烯酰胺化的双重上游和下游元素

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Regulation of polyadenylation efficiency at the secretary poly(A) site plays an essential role in gene expression at the immunoglobulin (IgM) locus. At this poly(A) site the consensus AAUAAA hexanucleotide sequence is embedded in an extended AU-rich region and there are two downstream GU-rich regions which are suboptimally placed. As these sequences are involved in formation of the polyadenylation pre-initiation complex, we examined their function in vivo and in vitro. We show that the upstream AU-rich region can function in the absence of the consensus hexanucleotide sequence both in vivo and in vitro and that both GU-rich regions are necessary for full polyadenylation activity in vivo and for formation of polyadenylation-specific complexes in vitro. Sequence comparisons reveal that: (i) the dual structure is distinct for the IgM secretary poly(A) site compared with other immunoglobulin isotype secretary poly(A) sites; (ii) the presence of an AU-rich region close to the consensus hexanucleotide is evolutionarily conserved for IgM secretary poly(A) sites. We propose that the dual structure of the IgM secretary paly(A) site provides a flexibility to accommodate changes in polyadenylation complex components during regulation of polyadenylation efficiency.
机译：在秘书poly（A）位点的聚腺苷酸化效率的调节在免疫球蛋白（IgM）基因座的基因表达中起着至关重要的作用。在该多聚（A）位点，共有的AAUAAA六核苷酸序列被嵌入扩展的富含AU的区域，并且有两个下游的富含GU的区域被亚最佳地放置。由于这些序列参与了聚腺苷酸预起始复合物的形成，因此我们在体内和体外研究了它们的功能。我们表明，在体内和体外，在没有共有六核苷酸序列的情况下，上游富含AU的区域可以起作用，并且两个富含GU的区域对于体内完全的聚腺苷酸化活性和体外形成聚腺苷酸化特异性复合物都是必需的。序列比较显示：（i）与其他免疫球蛋白同型秘书多聚腺苷酸位点相比，IgM秘书多聚腺苷酸位点的二元结构是不同的; （ii）IgM秘书poly（A）位点在进化上保守了接近共有六核苷酸的富金区。我们建议IgM秘书paly（A）网站的双重结构提供了灵活性，以适应在调节聚腺苷酸化效率的过程中聚腺苷酸化复合物组分的变化。

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