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首页> 外文期刊>Nucleic Acids Research >KLUYVEROMYCES LACTIS KILLER SYSTEM - ANALYSIS OF CYTOPLASMIC PROMOTERS OF THE LINEAR PLASMIDS
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KLUYVEROMYCES LACTIS KILLER SYSTEM - ANALYSIS OF CYTOPLASMIC PROMOTERS OF THE LINEAR PLASMIDS

机译：克鲁维酵母乳酸菌杀手系统-线性质粒的细胞质启动子分析

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摘要

Ail of the 14 genes encoded by the cytoplasmic linear killer plasmids of Kluyveromyces lactis are preceded by upstream conserved sequences (UCSs), cis-acting elements involved in plasmid gene transcription, Using the bacterial glucose-dehydrogenase gene as a reporter, expression driven by seven cytoplasmic promoters was determined. The level of expression ranged from 0.5 to 6 nkat, The highest activity was displayed by UCS 6 of pGKL2 whereas the lowest level was obtained with UCS2 of pGKL2, all other values were in between, Sequences located 5' upstream the UCSs do not influence expression, As exemplified for UCS5 and UCS10, deletion led to an almost complete loss of expression.
机译：乳酸克鲁维酵母细胞质线性杀手质粒编码的14个基因中的所有基因之前都有上游保守序列（UCS），参与质粒基因转录的顺式作用元件，以细菌葡萄糖脱氢酶基因为报告基因，由7个基因驱动的表达确定了细胞质启动子。表达水平的范围是0.5到6 nkat，pGKL2的UCS 6显示了最高的活性，而pGKL2的UCS2显示了最低的活性，所有其他值都在中间，位于UCS上游5'的序列不影响表达。如针对UCS5和UCS10所例示的，缺失导致表达几乎完全丧失。

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