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首页> 外文期刊>Nucleic Acids Research >Fusions with histone H3 result in highly specific alteration of gene expression.
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Fusions with histone H3 result in highly specific alteration of gene expression.

机译：与组蛋白H3的融合导致基因表达的高度特异性改变。

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摘要

Hap1 is a yeast transcriptional activator which controls expression of genes such as CYC1 and CYC7. Our results show that Hap1 activity is dependent on a functional chromatin remodeling complex SWI/SNF. Using a modified two-hybrid screen with Hap1 as bait, we recovered expression vectors encoding the Gal4 activation domain fused to histone H3 [Gal4(AD)-H3]. Hap1 activity at CYC1 or CYC7 was increased by Gal4(AD)-H3 and the effect was dependent on the presence of the activation domain of Hap1 and a functional SWI complex. Importantly, overexpression of H3 alone had no effect on Hap1 activity. Analysis of Gal4(AD)-H3 revealed that the fusion is not incorporated into the nucleosome while a functional Gal4 activation domain is dispensable. Activity of many other transcriptional activators was unchanged or slightly affected in the presence of Gal4(AD)-H3. Thus, our results identify a new class of histone H3 variants that cause highly specific alteration of gene expression. Hap1 may interact directly with H3 favoring chromatin remodeling by the SWI/SNF complex.
机译：Hap1是一种酵母转录激活因子，可控制CYC1和CYC7等基因的表达。我们的结果表明，Hap1活性取决于功能性染色质重塑复合体SWI / SNF。使用改良的以Hap1为诱饵的双杂交筛选，我们回收了编码与组蛋白H3 [Gal4（AD）-H3]融合的Gal4激活域的表达载体。 Gal4（AD）-H3可增加CYC1或CYC7的Hap1活性，其作用取决于Hap1激活域和功能性SWI复合物的存在。重要的是，仅H3的过表达对Hap1活性没有影响。对Gal4（AD）-H3的分析表明，融合是不掺入核小体中的，而功能性Gal4激活域是可有可无的。在Gal4（AD）-H3的存在下，许多其他转录激活因子的活性未改变或受到轻微影响。因此，我们的结果确定了导致基因表达高度特异性改变的一类新的组蛋白H3变体。 Hap1可能直接与H3相互作用，从而促进SWI / SNF复合物对染色质的重塑。

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