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首页> 外文期刊>Cell metabolism >Detection and Quantitation of Circulating Human Irisin by Tandem Mass Spectrometry
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Detection and Quantitation of Circulating Human Irisin by Tandem Mass Spectrometry

机译：串联质谱法检测和定量循环中的人鸢尾素

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摘要

Exercise provides many health benefits, including improved metabolism, cardiovascular health, and cognition. We have shown previously that FNDC5, a type I transmembrane protein, and its circulating form, irisin, convey some of these benefits in mice. However, recent reports questioned the existence of circulating human irisin both because human FNDC5 has a non-canonical ATA translation start and because of claims that many human irisin antibodies used in commercial ELISA kits lack required specificity. In this paper we have identified and quantitated human irisin in plasma using mass spectrometry with control peptides enriched with heavy stable isotopes as internal standards. This precise state-of-the-art method shows that human irisin is mainly translated from its non-canonical start codon and circulates at similar to 3.6 ng/ml in sedentary individuals; this level is increased to similar to 4.3 ng/ml in individuals undergoing aerobic interval training. These data unequivocally demonstrate that human irisin exists, circulates, and is regulated by exercise.
机译：运动可带来许多健康益处，包括改善新陈代谢，心血管健康和认知。先前我们已经证明FNDC5（一种I型跨膜蛋白）及其循环形式虹膜素在小鼠中传达了其中一些益处。但是，最近的报道质疑循环的人类虹膜素的存在，这既是因为人类FNDC5具有非规范的ATA翻译起点，又是因为声称用于商业ELISA试剂盒的许多人类虹膜素抗体缺乏所需的特异性。在本文中，我们已经使用质谱法鉴定和定量了血浆中的人虹膜素，并以富含重度稳定同位素的对照肽作为内标。这种精确的最新技术表明，人虹膜素主要从其非规范起始密码子翻译而来，在久坐的人群中的循环速率约为3.6 ng / ml。在进行有氧间歇训练的个体中，该水平增加到类似于4.3 ng / ml。这些数据清楚地表明，人体虹膜素存在，循环并受锻炼调节。

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