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首页> 外文期刊>Cell motility and the cytoskeleton >Lasp-2 expression, localization, and ligand interactions: a new Z-disc scaffolding protein.
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Lasp-2 expression, localization, and ligand interactions: a new Z-disc scaffolding protein.

机译：Lasp-2表达，定位和配体相互作用：一种新型的Z盘支架蛋白。

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摘要

The nebulin family of actin-binding proteins plays an important role in actin filament dynamics in a variety of cells including striated muscle. We report here the identification of a new striated muscle Z-disc associated protein: lasp-2 (LIM and SH3 domain protein-2). Lasp-2 is the most recently identified member of the nebulin family. To evaluate the role of lasp-2 in striated muscle, lasp-2 gene expression and localization were studied in chick and mouse tissue, as well as in primary cultures of chick cardiac and skeletal myocytes. Lasp-2 mRNA was detected as early as chick embryonic stage 25 and lasp-2 protein was associated with developing premyofibril structures, Z-discs of mature myofibrils, focal adhesions, and intercalated discs of cultured cardiomyocytes. Expression of GFP-tagged lasp-2 deletion constructs showed that the C-terminal region of lasp-2 is important for its localization in striated muscle cells. Lasp-2 organizes actin filaments into bundles and interacts directly with the Z-disc protein alpha-actinin. These results are consistent with a function of lasp-2 as a scaffolding and actin filament organizing protein within striated muscle Z-discs.
机译：肌动蛋白结合蛋白的星云蛋白家族在包括横纹肌在内的多种细胞的肌动蛋白丝动力学中起着重要作用。我们在这里报告了一种新的横纹肌Z盘相关蛋白的鉴定：lasp-2（LIM和SH3域蛋白2）。 Lasp-2是最近鉴定的星云蛋白家族成员。为了评估lasp-2在横纹肌中的作用，研究了lasp-2基因在鸡和小鼠组织中以及鸡心肌和骨骼肌原代培养物中的表达和定位。 Lasp-2 mRNA最早在雏鸡胚胎第25阶段就被检测到，而lasp-2蛋白与发育的肌原纤维结构，成熟的肌原纤维的Z盘，粘着斑和培养的心肌细胞盘之间存在联系。 GFP标记的lasp-2缺失构建体的表达表明，lasp-2的C端区域对于其在横纹肌细胞中的定位很重要。 Lasp-2将肌动蛋白丝组织成束，并直接与Z-disc蛋白α-肌动蛋白相互作用。这些结果与lasp-2作为横纹肌Z盘中的支架和肌动蛋白丝组织蛋白的功能一致。

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