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首页> 外文期刊>Cell motility and the cytoskeleton >Actin cytoskeleton reorganization of the apoptotic nurse cells during the late developmental stages of oogenesis in Dacus oleae.
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Actin cytoskeleton reorganization of the apoptotic nurse cells during the late developmental stages of oogenesis in Dacus oleae.

机译：茶树卵子发育的后期发育阶段，凋亡护士细胞的肌动蛋白细胞骨架重组。

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In the present study, we demonstrate the actin cytoskeleton reorganization during nurse cells apoptosis of the olive fruit fly Dacus oleae. At the developmental stage 9A of oogenesis, the actin microfilaments are assembled in numerous ring canals and subcortically support all the nurse cells, as is shown by phalloidin-FITC staining. During the following stages, 9B and 10A, this structural pattern remains the same. The developmental stage 10B is characterized by actin microfilament rearrangement and formation of actin cables that are symmetrically organized around the nurse cell nuclei. At stage 11, when the dumping process begins, these actin cables seem to retain each nurse cell nucleus in the cell center, away from blocking the ring canals. The early stage 12 is characterized by an asynchronous nurse cell nuclear chromatin condensation, while at late stage 12 the actin cables become very thick, as adjacent ones overlap one another and traverse the disorganized apoptotic nurse cell nuclei that already have fragmented DNA, as is demonstrated by acridine orange staining and TUNEL assay. Finally, during stage 13, the apoptotic nuclear remnants are phagocytosed by the neighboring follicle cells. The data presented herein compared to previous reported results in Drosophila [Nezis et al., 2000: Eur J Cell Biol 79:610-620], demonstrate that actin cytoskeleton reorganization during nurse cell apoptosis is a developmentally regulated physiological mechanism, phylogenetically conserved in higher Dipteran. Copyright 2001 Wiley-Liss, Inc.
机译：在本研究中，我们证明了橄榄实蝇Dacus oleae的护士细胞凋亡过程中的肌动蛋白细胞骨架重组。在卵子发生的发育阶段9A，肌动蛋白微丝组装在许多环管中，并皮质下支持所有护士细胞，如鬼笔环肽-FITC染色所示。在随后的阶段9B和10A中，此结构模式保持不变。发育阶段10B的特征在于肌动蛋白微丝重排和肌动蛋白电缆的形成，其围绕护士细胞核对称地组织。在第11阶段，当倾销过程开始时，这些肌动蛋白电缆似乎将每个护士细胞核保留在细胞中心，而不会阻塞环管。如图所示，第12阶段的早期特征是异步护士细胞核染色质凝结，而在第12阶段后期，肌动蛋白电缆变得很粗，因为相邻的肌动蛋白电缆相互重叠并穿过已经断裂的DNA的无序的凋亡护士细胞核。通过a啶橙染色和TUNEL测定。最终，在第13阶段中，凋亡的核残余物被邻近的卵泡细胞吞噬。本文提供的数据与果蝇先前报道的结果进行了比较[Nezis et al。，2000：Eur J Cell Biol 79：610-620]，表明护士细胞凋亡期间肌动蛋白的细胞骨架重组是发育调节的生理机制，在更高的系统发育上是保守的。 Dipteran。版权所有2001 Wiley-Liss，Inc.

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