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首页> 外文期刊>Lab on a chip >On-chip pumping for pressure mobilization of the focused zones following microchip isoelectric focusing
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On-chip pumping for pressure mobilization of the focused zones following microchip isoelectric focusing

机译：芯片上泵浦以在微芯片等电点聚焦后动员聚焦区域的压力

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摘要

Isoelectric focusing (IEF),traditionally accomplished in slab or tube gels,has also been performed extensively in capillary and,more recently,in microchip formats.IEF separations performed in microchips typically use electroosmotic flow (EOF) or chemical treatment to mobilize the focused zones past the detection point.This report describes the development and optimization of a microchip IEF method in a hybrid PDMS-glass device capable of controlling the mobilization of the focused zones past the detector using on-chip diaphragm pumping.The microchip design consisted of a glass fluid layer (separation channels),a PDMS layer and a glass valve layer (pressure connections and valve seats).Pressure mobilization was achieved on-chip using a diaphragm pump consisting of a series of reversible elastomeric valves,where a central diaphragm valve determined the volume of solution displaced while the gate valves on either side imparted directionality.The pumping rate could be adjusted to control the mobilization flow rate by varying the actuation times and pressure applied to the PDMS to actuate the valves.In order to compare the separation obtained using the chip with that obtained in a capillary,a serpentine channel design was used to match the separation length of the capillary,thereby evaluating the effect of diaphragm pumping itself on the overall separation quality.The optimized mIEF method was applied to the separation of labeled amino acids.
机译：等电聚焦（IEF）传统上是在平板或管凝胶中完成的，也已广泛用于毛细管和最近的微芯片形式。在微芯片中进行的IEF分离通常使用电渗流（EOF）或化学处理来移动聚焦区域本报告介绍了混合PDMS玻璃设备中微芯片IEF方法的开发和优化，该设备可使用片上隔膜泵控制通过探测器的聚焦区域的移动。微芯片设计由玻璃组成流体层（分离通道），PDMS层和玻璃阀层（压力连接和阀座）。使用由一系列可逆弹性阀组成的隔膜泵在芯片上实现压力动员，其中中央隔膜阀确定当两侧的闸阀赋予方向性时，溶液的排量可以调节。可以调节抽速以控制温度为了通过改变施加在PDMS上的致动时间和压力来动员流速，以致动阀门。毛细管，从而评价隔膜泵本身对整体分离质量的影响。优化的mIEF方法用于标记氨基酸的分离。

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