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首页> 外文期刊>Laboratory Animal Science >STEADY-STATE MINUTE VOLUME DETERMINATION BY BODY-ONLY PLETHYSMOGRAPHY IN JUVENILE RHESUS MONKEYS
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STEADY-STATE MINUTE VOLUME DETERMINATION BY BODY-ONLY PLETHYSMOGRAPHY IN JUVENILE RHESUS MONKEYS

机译：幼体猴的体内体态图测定稳态分钟体积

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When one is using nonhuman primates for studying the inhalation of infective or toxic agents, a respiratory minute volume (MV) range of +/-50 ml is desirable to ensure the accurate delivery of calculated doses of the aerosolized agent, When one is working with highly infective or toxic agents, it is desirable to anesthetize the animals and to separate the plethysmograph, used to measure MV, from the aerosol chamber, used to administer agents, in order to minimize decontamination procedures and to maximize safety, In our laboratory the sequential completion of these procedures requires at least 20 min. Therefore it is necessary to find an anesthetic that achieves a +/-50 ml steady-state MV for at least 20 min and that does not change when an animal is transported from one apparatus to another, Using 2.6- to 4.0-kg, 14- to 18-month-old rhesus macaques, we determined that tiletamine/zolazepam induced a steady-state MV of 48+/-17.8 min, beginning 21.5+/-4.7 min after injection of the anesthetic agent, This MV did not significantly change when animals were transported, The use of ketamine and ketamine/acepromazine resulted in a steady-state MV period of 11.5+/-4.5 and 22.0+/-7.9 min respectively. When we compared these findings with previously reported mathematical estimations of MV based on functions of weight or respiratory rate, we further determined that the accurate measurement of MV before each aerosol exposure was critical for calculating inhaled doses of the agent.
机译：当使用非人类灵长类动物研究传染性或有毒物质的吸入时，理想的呼吸分钟体积（MV）范围为+/- 50 ml，以确保准确递送雾化剂的计算剂量。为了对动物进行麻醉，并将用于测量MV的体积描记器与用于施药的气溶胶室分开，以减少去污程序并最大程度地提高安全性，在我们的实验室中完成这些步骤至少需要20分钟。因此，有必要找到一种麻醉剂，该麻醉剂至少在20分钟内达到+/- 50 ml的稳态MV，并且在使用2.6-4.0 kg的动物从一种设备运输到另一种设备时不改变麻醉剂，14 -对于18个月大的恒河猕猴，我们确定，在注射麻醉剂后21.5 +/- 4.7分钟开始，tiletamine / zolazepam诱导的稳态MV为48 +/- 17.8分钟，该MV没有明显变化当运输动物时，使用氯胺酮和氯胺酮/乙酰丙嗪分别导致稳态MV期为11.5 +/- 4.5分钟和22.0 +/- 7.9分钟。当我们将这些发现与先前报道的基于体重或呼吸频率函数的MV的数学估计值进行比较时，我们进一步确定，每次气雾暴露之前MV的准确测量对于计算药物的吸入剂量至关重要。

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