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首页> 外文期刊>Laboratory Animal Science >SWR: an inbred strain suitable for generating transgenic mice.
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SWR: an inbred strain suitable for generating transgenic mice.

机译：SWR：适于产生转基因小鼠的近交系。

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The production of fertilized oocytes and generation of transgenic mice is generally more efficient using F2 hybrid embryos than embryos from inbred mice. Most F2 hybrids are of the C57BL/6 background because of its genetic and embryological features. However, in order to develop a transgenic mouse model for rheumatoid arthritis, a susceptible mouse strain such as DBA/1 had to be used to produce transgenics. Gene constructs containing the alpha and beta T-cell receptor (TCR) chain were microinjectedinto embryos from DBA/1, SWR, (DBA/1 X SWR)F1 and (SWR X DBA/1)F1 strains. SWR female mice were found to be prolific ovulators in response to exogenous hormones, with oocyte numbers comparable with those produced by (C57BL/6 X C3H)F1 female mice. Embryos from the (SWR X DBA/1)F1 or SWR strain were large and had prominent pronuclei, whereas (DBA/1 X SWR)F1 embryos were smaller and had less visible pronuclei, similar to those of DBA/1 embryos. Therefore, the pronuclear size and visibility were features of the SWR female mice and were independent of the genotype of the fertilizing spermatozoa. Resistance to lysis after co-injection of alphabeta TCR constructs and the efficiency of generating DNA-positive founders were comparable in SWR, (SWR X DBA/1)F1,and (C57BL6 X C3H)F2 embryos. It is concluded that the SWR mouse is another inbred strain, in addition to the FVB inbred strain, which is highly suitable for the propagation of transgenes.
机译：使用F2杂种胚胎比自交小鼠的胚胎通常更有效地生产受精卵母细胞和转基因小鼠。由于其遗传和胚胎学特征，大多数F2杂种具有C57BL / 6背景。但是，为了开发类风湿关节炎的转基因小鼠模型，必须使用易感小鼠品系（例如DBA / 1）来产生转基因。将含有α和βT细胞受体（TCR）链的基因构建体显微注射到DBA / 1，SWR，（DBA / 1 X SWR）F1和（SWR X DBA / 1）F1菌株的胚胎中。发现SWR雌性小鼠是对外源激素作出反应的多产排卵者，卵母细胞数量可与（C57BL / 6 X C3H）F1雌性小鼠产生的卵母细胞数量相当。 （SWR X DBA / 1）F1或SWR株的胚大而有突出的前核，而（DBA / 1 X SWR）F1胚更小并且有较少的可见核，类似于DBA / 1胚胎。因此，前核的大小和可见度是SWR雌性小鼠的特征，并且与受精精子的基因型无关。在SWR，（SWR X DBA / 1）F1和（C57BL6 X C3H）F2胚胎中，共注射安捷伦TCR构筑物后对裂解的抗性和产生DNA阳性创建者的效率相当。结论是，除了FVB近交系外，SWR小鼠是另一近交系，其非常适合转基因的繁殖。

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