• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell motility and the cytoskeleton >Dynamic distribution of an antigen involved in the differentiation of avian myoblasts: II. Possible association of beta1 integrin with myofibril organization.
【24h】

Dynamic distribution of an antigen involved in the differentiation of avian myoblasts: II. Possible association of beta1 integrin with myofibril organization.

机译：涉及禽成肌细胞分化的抗原的动态分布：II。 beta1整合素可能与肌原纤维组织相关。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previous studies have shown that a monoclonal antibody, H-145, inhibits myotube formation of quail myoblasts transformed with a temperature-sensitive mutant of Rous sarcoma virus (QM-RSV cells) [Hyodo and Kim, 1994: Exp. Cell Res. 212:120-131]. The antigen recognized by H-145 (H-145 antigen), which is a glycoprotein with a molecular mass of about 116 kDa, is related to a step immediately before myoblast fusion. To determine the functional significance of H-145 antigen, we examined its dynamic state during myogenic differentiation of QM-RSV cells. H-145 antigen showed a unique and discrete distribution. In immature myotubes immediately after myoblast fusion, many ring-like structures of H-145 antigen appeared on the ventral surface of the cells, encircling the actin dots detected simultaneously by immunofluorescence and interference reflection microscopy. The core of the ring-like structures was filled with the termini of actin bundles, mainly formed by alpha-actin. Other cytoskeletal-associated proteins, such as vinculin and alpha-actinin, were also associated with these structures. The ring-like structures of H-145 antigen were observed only during a restricted period when myoblasts fused actively, suggesting their relationships to myotube formation and an early stage of myofibril formation. With maturation of the myotubes, most of the H-145 antigen became redistributed in linear arrays on the apical cell surface and was probably associated with the termini of actin bundles to organize myofibrils, suggesting that the antigen was also related to maturation of myotubes. Experiments using monoclonal antibodies against chick beta1 integrin showed that H-145 antigen is beta1 integrin or a very closely related derivation. Thus H-145 antigen (beta1 integrin) is possibly involved in both myoblast fusion and the myofibril organization in myotubes. Copyright 2000 Wiley-Liss, Inc.
机译：先前的研究表明，单克隆抗体H-145抑制由劳斯肉瘤病毒（QM-RSV细胞）的温度敏感突变体转化的鹌鹑成肌细胞的肌管形成[Hyodo and Kim，1994：Exp。 Cell Res。 212：120-131]。 H-145识别的抗原（H-145抗原）是一种分子量约为116 kDa的糖蛋白，与成肌细胞融合之前的步骤有关。为了确定H-145抗原的功能意义，我们检查了QM-RSV细胞成肌分化过程中的动态状态。 H-145抗原显示出独特且离散的分布。在成肌细胞融合后未成熟的肌管中，H-145抗原的许多环状结构出现在细胞的腹表面，包围了通过免疫荧光和干扰反射显微镜同时检测到的肌动蛋白点。环状结构的核心充满了肌动蛋白束末端，肌动蛋白束主要由α-肌动蛋白形成。其他与细胞骨架相关的蛋白质，例如长春菊酯和α-肌动蛋白，也与这些结构相关。 H-145抗原的环状结构仅在成肌细胞主动融合的有限时间内被观察到，这表明它们与肌管形成和肌原纤维形成的早期有关。随着肌管的成熟，大多数H-145抗原重新分布在顶端细胞表面的线性阵列中，并可能与肌动蛋白束的末端相关，从而组织了肌原纤维，这表明该抗原也与肌管的成熟有关。使用针对雏鸡beta1整合素的单克隆抗体进行的实验表明，H-145抗原是beta1整合素或非常密切相关的衍生物。因此，H-145抗原（β1整合素）可能参与成肌细胞融合和肌管中的肌原纤维组织。版权所有2000 Wiley-Liss，Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号