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首页> 外文期刊>Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis >Generation of cytotoxic donor CD8+ T cells against relapsing leukemic cells following allogeneic transplantation by stimulation with leukemic cell- or leukemic lysate pulsed donor cell-derived dendritic cells.
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Generation of cytotoxic donor CD8+ T cells against relapsing leukemic cells following allogeneic transplantation by stimulation with leukemic cell- or leukemic lysate pulsed donor cell-derived dendritic cells.

机译：通过用白血病细胞或白血病裂解物脉冲的供体细胞衍生的树突状细胞刺激，进行异体移植后针对复发性白血病细胞的细胞毒性供体CD8 + T细胞的产生。

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摘要

To treat leukemia relapse after allogeneic hematopoietic stem cell transplantation (HSCT), we investigated the possibility of immunotherapy using donor CD8+ T cells that were generated by stimulating leukemic cell-derived dendritic cells (leukemic-DCs) or leukemic cell lysate pulsed donor cell-derived DCs (donor-DCs). Leukemic- and donor-DCs were generated from mononuclear cells of patients and CD14+ cells of HLA-matched donors, respectively. The expression of CD80, CD83, CD86, CD1a, and CD40 on leukemic-DCs was significantly lower than that on donor-DCs. Donor-DCs exhibited a higher capacity to stimulate allogeneic T cells compared with leukemic-DCs. Donor CD8+ T cells stimulated by leukemic- or donor-DCs were more cytotoxic than unprimed CD8+ T cells, and slightly higher cytotoxicity was observed with donor-DCs compared to leukemic-DCs. This study indicates that leukemic- or donor-DCs pulsed with leukemic cell lysates can effectively prime donor cytotoxic T cells in vitro, and that they may be used as a potential alternative tool for treating leukemic patients who relapse after allogeneic HSCT.
机译：为了治疗同种异体造血干细胞移植（HSCT）后的白血病复发，我们研究了使用通过刺激白血病细胞衍生的树突状细胞（leukmic-DCs）或白血病细胞裂解液脉冲后的供体细胞衍生的供体CD8 + T细胞进行免疫疗法的可能性DC（捐助方DC）。分别从患者的单核细胞和HLA匹配的供体的CD14 +细胞生成白血病DC和供体DC。 CD80，CD83，CD86，CD1a和CD40在白血病DC上的表达明显低于供体DC。与白血病DC相比，供体DC表现出更高的刺激同种异体T细胞的能力。白血病或供体DC刺激的供体CD8 + T细胞比未引发的CD8 + T细胞具有更高的细胞毒性，并且与白血病DC相比，供体DC观察到的细胞毒性稍高。这项研究表明，用白血病细胞裂解液脉冲的白血病或供体DC可以在体外有效地引发供体细胞毒性T细胞，并且它们可以用作治疗异基因HSCT后复发的白血病患者的潜在替代工具。

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