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首页> 外文期刊>Cell biology international. >Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow.
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Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow.

机译:与骨髓的其他亚群相比,间充质干细胞显示出受损肝组织再生的最高潜力。

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We have previously reported that bone marrow cells (BMCs) participate in the regeneration after liver injury. However, it is not established that this is the result of differentiation of hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) or the combination of both. We investigated the contribution of each cell fraction to the regenerative process. First, we confirmed that transplanted stem cells migrate directly to injured liver tissue without dispersing to other organs. Next, we divided green fluorescent protein (GFP)-expressing BMCs into three populations as mononuclear cells, MSCs and HSCs. We then compared the engraftment capacity after transplantation of each fraction of cells into liver-injured mice. Of these, the MSCs transplanted group showed the highest GFP fluorescence intensities in liver tissue by flow cytometry analysis and confocal microscopic observation. Furthermore, MSCs showed differentiation potential into hepatocytes when co-cultured with injured liver cells, which suggests that MSCs showed highest potential for the regeneration of injured liver tissue compared with those of the other two cell refractions.
机译:我们以前曾报道过,骨髓细胞(BMC)参与肝损伤后的再生。但是,尚不能确定这是造血干细胞(HSC),间充质干细胞(MSC)或两者结合的结果。我们调查了每个细胞部分对再生过程的贡献。首先,我们确认移植的干细胞直接迁移到受损的肝组织,而不会扩散到其他器官。接下来,我们将表达绿色荧光蛋白(GFP)的BMC分为三个群体,即单核细胞,MSC和HSC。然后,我们比较了将各部分细胞移植到肝损伤小鼠体内后的植入能力。其中,通过流式细胞术分析和共聚焦显微镜观察,MSCs移植组在肝组织中显示出最高的GFP荧光强度。此外,当与受损肝细胞共培养时,MSCs显示出分化为肝细胞的潜力,这表明与其他两种细胞折射相比,MSCs显示出了受损肝脏组织再生的最高潜力。

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