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首页> 外文期刊>Cell motility and the cytoskeleton >Identification of a novel actin-related protein in Tetrahymena cilia.
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Identification of a novel actin-related protein in Tetrahymena cilia.

机译：在纤毛膜纤毛中鉴定一种新型肌动蛋白相关蛋白。

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摘要

Actin is an ancient cytoskeletal protein that plays many essential roles in cell motility. In eukaryotes, its gene belongs to a highly conserved gene family, while the protein is also a member of an actin superfamily comprising various kinds of actin-related proteins (Arps). A ciliate, Tetrahymena, has a unique conventional actin. Data from the TIGR Tetrahymena genome project and our own research suggest the existence of 12 actin-like sequences: four conventional actins, two of Arp4, one each of Arp1, Arp2, Arp3, Arp5, and Arp6, and a novel actin-related protein, tArp. We cloned the entire cDNA sequences of Tetrahymena Arp2 (tArp2), Tetrahymena Arp3 (tArp3), and tArp for the work described herein. In phylogenetic analyses, tArp was not included in any Arp subfamily. Unlike other known Arps, tArp localizes in cilia, and its expression was upregulated after deciliation. To see the precise localization of tArp, cilia were fractionated and analyzed using specific antibodies. tArp was observed preferentially in the "outer-doublet" fraction, while actin was found in the "crude-dynein" fraction. In immunoelectron microscopy, most of the gold particles were found either on the outer-doublet or central-pair microtubules. These results suggest that tArp is a ciliary component and that it has a unique function in the formation and maintenance of cilia.
机译：肌动蛋白是一种古老的细胞骨架蛋白，在细胞运动中起许多重要作用。在真核生物中，其基因属于高度保守的基因家族，而该蛋白还是肌动蛋白超家族的成员，肌动蛋白超家族包括各种肌动蛋白相关蛋白（Arps）。纤毛虫，四膜虫，具有独特的常规肌动蛋白。 TIGR四膜虫基因组计划的数据和我们自己的研究表明存在12种肌动蛋白样序列：四种常规肌动蛋白，两种Arp4，每种Arp1，Arp2，Arp3，Arp5和Arp6以及一种新型肌动蛋白相关蛋白，tArp。对于本文所述的工作，我们克隆了四膜虫Arp2（tArp2），四膜虫Arp3（tArp3）和tArp的完整cDNA序列。在系统发育分析中，任何Arp亚科均不包含tArp。与其他已知的Arps不同，tArp定位在纤毛中，其表达在减退后上调。为了观察tArp的精确定位，对纤毛进行了分级，并使用特异性抗体进行了分析。优先在“双-双峰”部分中观察到tArp，而在“粗制动力蛋白”部分中发现肌动蛋白。在免疫电子显微镜中，大多数金颗粒都在外双峰或中心对微管上发现。这些结果表明，tArp是纤毛成分，并且在纤毛的形成和维持中具有独特的功能。

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