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首页> 外文期刊>Cell motility and the cytoskeleton >Functional binding of inner-arm dyneins with demembranated flagella of Chlamydomonas mutants.
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Functional binding of inner-arm dyneins with demembranated flagella of Chlamydomonas mutants.

机译：手臂动力蛋白与衣藻衣原体去膜鞭毛的功能结合。

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Experiments were carried out to see if isolated inner arm dyneins could functionally combine with axonemes lacking them. High-salt extract from the axoneme of Chlamydomonas oda1 mutant lacking outer-arm dynein was added to the demembranated cell models of ida1oda1 lacking inner arm dynein f (dynein I1) and outer arm dynein. After incubation, the originally paralyzed ida1oda1 axonemes recovered the ability to beat in the presence of ATP. A similar good motility recovery after incubation with crude oda1 extract was observed in ida9oda2 lacking outer arm and inner arm dynein c, and partial recovery in ida4oda1 lacking outer arm and inner arm species a, c, and d. These observations indicate that dynein f and dynein c can functionally bind with mutant axonemes lacking them. A method for combining isolated inner arm dyneins with axonemes in a functionally active manner should provide a powerful experimental tool with which to study the mechanism of beating.
机译：进行了实验，以查看孤立的内臂动力蛋白是否可以与缺乏它们的轴突蛋白功能结合。从衣藻衣原体缺乏外臂动力蛋白的轴突酶的高盐提取物添加到缺乏内臂动力蛋白f（dynein I1）和外臂动力蛋白的ida1oda1的脱膜细胞模型中。孵育后，最初瘫痪的ida1oda1轴索蛋白恢复了在ATP存在下的搏动能力。与缺少粗大的oda1提取物一起孵育后，在缺少外臂和内臂动力蛋白c的ida9oda2中观察到了类似的良好运动恢复，而在缺少外臂和内臂物种a，c和d的ida4oda1中部分恢复。这些观察结果表明，动力蛋白f和动力蛋白c可以与缺乏它们的突变轴蛋白功能性结合。一种以功能活跃的方式将孤立的内臂动力蛋白与轴突结合的方法，应提供一个强大的实验工具，用以研究跳动的机理。

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