• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Effects of colchicine or demecolcine on cytoplasmic protrusions and assisted enucleation of golden hamster oocytes.
【24h】

Effects of colchicine or demecolcine on cytoplasmic protrusions and assisted enucleation of golden hamster oocytes.

机译：秋水仙碱或地美可星对金黄仓鼠卵母细胞胞浆突起和去核的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To establish experimental protocols for cloning golden hamsters, optimal concentrations of colchicine and demecolcine were determined for inducing cytoplasmic protrusion (containing chromosomes) and assisting enucleation of their oocytes. Denuded oocytes at different ages were treated with 2.5-10 microg/ml of colchicine for 1-4h or 0.02-0.6 microg/ml of demecolcine for 15-60 min. Cytoplasmic protrusions of oocytes were removed with a micromanipulation pipette. The results show that: 1) at 13.5-18h post-hCG injection, approximately 90% of oocytes treated for with 10 microg/ml of colchicine formed cytoplasmic protrusions, and in some oocytes enucleation occurred; 2) when treated with 0.4 microg/ml of demecolcine for 1h, cytoplasmic protrusions 13.5-18h post-hCG treatment were present in almost all oocytes; 3) after the protrusions induced by either treatment had been removed, the assisted enucleation rate was >80%, whereas it was approximately 32% with blind enucleation.
机译：为了建立克隆金黄仓鼠的实验方案，确定了最佳浓度的秋水仙碱和地美可辛，以诱导胞质突起（含有染色体）并协助其卵母细胞去核。用2.5-10微克/毫升秋水仙碱处理1-4h或0.02-0.6微克/毫升地美考克碱处理不同年龄的裸露卵母细胞15-60分钟。用微量移液管去除卵母细胞的胞质突起。结果表明：1）hCG注射后13.5-18h，约有90％经10μg/ ml秋水仙碱处理的卵母细胞形成细胞质突起，并且在某些卵母细胞中发生了去核。 2）当用0.4微克/毫升的地美可辛处理1小时时，hCG处理后13.5-18小时的细胞质突起几乎存在于所有卵母细胞中; 3）去除任何一种治疗引起的突起后，辅助摘除率> 80％，而盲摘除则约为32％。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号