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首页> 外文期刊>Cell biology international. >Hyperlipidemia induces vascular smooth muscle cell proliferation involving Wnt/beta-catenin signaling
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Hyperlipidemia induces vascular smooth muscle cell proliferation involving Wnt/beta-catenin signaling

机译：高脂血症诱导涉及Wnt /β-catenin信号传导的血管平滑肌细胞增殖

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摘要

Hyperlipidemia has been shown to stimulate vascular smooth muscle cell (VSMC) proliferation. Wnt signaling pathway plays a critical role in embryonic development and cell proliferation. In this study, Sprague-Dawley rats fed with high-fat or normal diet for 12 weeks were sacrificed, and the thoracic aorta was harvested to determine wnt3a, beta-catenin, T-cell factor 4 (TCF4), and cyclin D1 expressions. VSMC proliferation within thoracic aorta and lipid accumulation within VSMCs were detected. Rat aortic VSMCs were cultured in serum from rats with hyperlipidemia or DKK-1; Wnt3a, beta-catenin, TCF4, and cyclin D1 expressions, and cell cycle distribution were determined. The findings demonstrated that increased number of VSMCs, lipid droplets, and vacuoles within thoracic aorta in the high-fat-fed group. Compared with controls, VSMCs from high-fat-fed rats showed higher mRNA expressions of wnt3a, beta-catenin, TCF4, and cyclin D1, as well as in VSMCs cultured with hyperlipidemic serum. After 24 h, VSMCs stimulated with hyperlipidemic serum showed significantly increased cell number and S-phase entry compared with cells exposed to normolipidemic serum. These effects were blocked by DKK-1. These results suggest that Wnt/beta-catenin signaling plays an important role in hyperlipidemia-induced VSMC proliferation.
机译：高脂血症已显示可刺激血管平滑肌细胞（VSMC）增殖。 Wnt信号通路在胚胎发育和细胞增殖中起关键作用。在这项研究中，处死高脂或正常饮食喂养12周的Sprague-Dawley大鼠，并收集胸主动脉以确定wnt3a，β-catenin，T细胞因子4（TCF4）和细胞周期蛋白D1的表达。检测到胸主动脉内的VSMC增殖和VSMC内的脂质积聚。在高脂血症或DKK-1大鼠的血清中培养大鼠主动脉VSMC。确定了Wnt3a，β-catenin，TCF4和cyclin D1的表达以及细胞周期分布。研究结果表明，高脂饮食组胸主动脉内VSMC，脂质滴和液泡的数量增加。与对照组相比，高脂饮食大鼠的VSMC在wnt3a，β-catenin，TCF4和cyclin D1以及高血脂血清培养的VSMC中均具有较高的mRNA表达。 24小时后，与暴露于高血脂血清的细胞相比，用高血脂血清刺激的VSMC显示出明显增加的细胞数量和S期进入。这些作用被DKK-1阻断。这些结果表明，Wnt /β-catenin信号传导在高脂血症诱导的VSMC增殖中起重要作用。

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