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首页> 外文期刊>Cell and Tissue Research >Bone regeneration potential of allogeneic or autogeneic mesenchymal stem cells loaded onto cancellous bone granules in a rabbit radial defect model
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Bone regeneration potential of allogeneic or autogeneic mesenchymal stem cells loaded onto cancellous bone granules in a rabbit radial defect model

机译：兔radial骨缺损模型中异体或自体间充质干细胞加载到松质骨颗粒上的骨再生潜力

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摘要

For developing a clinically effective bone regeneration strategy, we compare the bone regeneration potential of cultured allogeneic bone marrow-derived mesenchymal stem cells (BM-MSCs) and of autologous BM-MSCs loaded onto allogeneic cancellous bone granule scaffolds. A critical-sized segmental bone defect was made at the mid-shaft of both radiuses in 19 New Zealand White rabbits (NWRs). In the experimental group, allogeneic BM-MSCs loaded onto small-sized allogeneic cancellous bone granules (300~700 um in diameter) were implanted in one side of a bone defect. In the control group, autologous BM-MSCs loaded onto allogeneic cancellous granules were grafted in the other side. Bone regeneration was assessed by radiographic evaluation at 4, 8, 12 and 16 weeks post-implantation and by micro-computed tomography (micro-CT) and histological evaluation at 8 and 16 weeks. The experimental groups showed lower bone quantity indices (BQIs) than the control groups at 12 and 16 weeks (p < 0.05), although no significant difference was observed at 4 and 8 weeks (p > 0.05). Micro-CT analysis revealed that both groups had similar mean total bone volume and other parameters including trabecular thickness, number and separation at either 8 or 16 weeks. Only bone surface area revealed less area in the experimental group at 16 weeks. Histological evaluation of 8-week and 16-week specimens showed similar biologic processes of new bone formation and maturation. There was no inflammatory reaction indicating an adverse immune response in both allogeneic and autologous MSC groups. In conclusion, allogeneic BM-MSCs loaded onto allogeneic cancellous bone granules had comparable bone regeneration potential to autologous BM-MSCs in a rabbit radial defect model.
机译：为了制定临床上有效的骨再生策略，我们比较了培养的同种异体骨髓来源的间充质干细胞（BM-MSC）和自体BM-MSC装载到同种异体松质骨颗粒支架上的骨再生潜力。在19只新西兰白兔（NWR）的两个radius骨的中轴上都形成了临界大小的节段性骨缺损。在实验组中，将同种异体BM-MSC植入小尺寸同种异体松质骨颗粒（直径300〜700 um）中，植入到骨缺损的一侧。在对照组中，将同种异体松质颗粒上的自体BM-MSCs移植到另一侧。在植入后第4、8、12和16周进行放射学评估，并在第8和16周进行显微计算机断层扫描（micro-CT）和组织学评估，以评估骨再生。实验组在第12周和第16周时显示出低于对照组的骨量指数（BQIs）（p <0.05），尽管在第4周和第8周时没有观察到显着差异（p> 0.05）。 Micro-CT分析显示，两组在8周或16周时的平均总骨体积和其他参数（包括小梁厚度，数量和分离度）均相似。在第16周，仅骨表面积在实验组中显示较少的面积。 8周和16周标本的组织学评估显示新骨形成和成熟的相似生物学过程。在同种异体和自体MSC组中均没有炎症反应表明免疫反应不良。总之，在兔radial骨缺损模型中，同种异体松质骨颗粒上装载的同种异体BM-MSC具有与自体BM-MSC相当的骨再生潜力。

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