• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Effect of IL-1 alpha at on the expression of cartilage matrix proteins in human chondrosarcoma cell line OUMS-27
【24h】

Effect of IL-1 alpha at on the expression of cartilage matrix proteins in human chondrosarcoma cell line OUMS-27

机译：IL-1α对人软骨肉瘤细胞OUMS-27软骨基质蛋白表达的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We examined the effect of the inflammatory mediator interleukin-1alpha (IL-1alpha) on cell proliferation, alkaline phosphatase (ALPase) activity, and the expressions of cartilage matrix proteins, bone morphogenetic protein-2 (BMP-2), and BMP-2 receptors in human chondrosarcoma cell line OUMS-27 (chondrocytes). The cells were cultured with Dulbecco's modified Eagle's medium containing 15% fetal bovine serum with 0, 1, 10, or 100 units/ml of IL-1alpha for up to 14 days. The expressions of cartilage matrix proteins, BMP-2, and BMP-2 receptors were estimated by determining mRNA levels using semiquantitative or real-time PCR and/or by determining protein levels using Enzyme-linked immunosorbent assay. Cell proliferation was decreased after 5 days in culture with IL-1alpha. The ALPase activity was decreased significantly in the presence of IL-1alpha until day 10 of culture. The expression of type 11 collagen was significantly decreased after 7 days in culture with IL-1alpha. The expressions of aggrecan and link protein were significantly decreased through day 14 of culture with EL-1alpha. The expression of BMP-2 was increased at days 3, 7, and 14 of culture with IL-1alpha, while the expression of type 11 receptor for BMP-2 was significantly decreased in the samples. These results suggest that IL-1alpha suppresses the expression of cartilage matrix proteins through a suppression of the autocrine action of BMP-2, brought about by the decrease in BMP-2 receptor expression in chondrocytes. (C) 2004 Elsevier Inc. All rights reserved.
机译：我们检查了炎症介质白介素1alpha（IL-1alpha）对细胞增殖，碱性磷酸酶（ALPase）活性以及软骨基质蛋白，骨形态发生蛋白2（BMP-2）和BMP-2表达的影响软骨肉瘤细胞系OUMS-27（软骨细胞）中的受体。将细胞用含有15％胎牛血清，0、1、10或100单位/ ml IL-1alpha的Dulbecco改良的Eagle's培养基培养最多14天。软骨基质蛋白，BMP-2和BMP-2受体的表达通过使用半定量或实时PCR测定mRNA水平和/或通过酶联免疫吸附测定来确定蛋白水平来估计。用IL-1α培养5天后，细胞增殖减少。在IL-1α存在下，直到培养的第10天，ALPase活性均显着降低。用IL-1alpha培养7天后，11型胶原蛋白的表达显着降低。通过用EL-1α培养的第14天，聚集蛋白聚糖和连接蛋白的表达显着降低。在用IL-1alpha培养的第3、7和14天，BMP-2的表达增加，而样品中BMP-2的11型受体的表达则明显降低。这些结果表明，IL-1α通过抑制BMP-2的自分泌作用来抑制软骨基质蛋白的表达，BMP-2的自分泌作用是由软骨细胞中BMP-2受体表达的降低引起的。 （C）2004 Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号