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Altered Bcl-2 and Bax expression is associated with cultured first trimester human cytotrophoblasts apoptosis induced by hypoxia

机译：Bcl-2和Bax表达的改变与缺氧诱导的孕早期人滋养细胞凋亡有关

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摘要

Preeclampsia is associated with placental hypoxia at early gestation. We therefore investigated the effect of hypoxia on the apoptosis of cultured first trimester human cytotrophoblasts and the expression of apoptosis relevant proteins, Bcl-2 and Bax. First trimester human cytotrophoblasts were isolated and cultured under either standard or hypoxic conditions. Cellular apoptosis was monitored by TUNEL and Annexin V binding, and the expression of Bcl-2 and Bax was determined by Western blot analysis. Apoptosis increased significantly in cytotrophoblasts cultured for 24 h under hypoxic conditions in contrast with those cultured under standard conditions, meanwhile expression of Bcl-2 reduced, and that of Bax increased. These changes suggested that hypoxia induced apoptosis in cultured first trimester cytotrophoblasts with altered Bcl-2 and Bax expression. Further study is needed to explore the role of cytotrophoblasts apoptosis in hypoxia-induced maternal and fetal diseases. (c) 2006 Elsevier Inc. All rights reserved.
机译：子痫前期与早期妊娠的胎盘缺氧有关。因此，我们研究了缺氧对培养的孕早期人滋养细胞凋亡以及凋亡相关蛋白Bcl-2和Bax表达的影响。分离出早孕的人类成细胞细胞，并在标准或低氧条件下培养。通过TUNEL和膜联蛋白V结合监测细胞凋亡，并通过Western印迹分析确定Bcl-2和Bax的表达。与标准条件下培养的细胞相比，低氧条件下培养24 h的细胞滋养细胞凋亡明显增加，而Bcl-2的表达减少，而Bax的表达增加。这些变化表明，低氧诱导培养的孕早期细胞滋养细胞凋亡，其Bcl-2和Bax表达改变。需要进一步研究以探索细胞滋养层细胞凋亡在缺氧诱导的母婴疾病中的作用。 （c）2006 Elsevier Inc.保留所有权利。

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