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首页> 外文期刊>Life sciences >Effect of berberrubine on interleukin-8 and monocyte chemotactic protein-1 expression in human retinal pigment epithelial cell line
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Effect of berberrubine on interleukin-8 and monocyte chemotactic protein-1 expression in human retinal pigment epithelial cell line

机译：小ber红素对人视网膜色素上皮细胞系白细胞介素8和单核细胞趋化蛋白-1表达的影响

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摘要

We examined the effects of berberrubine, a protoberberine alkaloid, on interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) expression in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin-1 beta (IL-1 beta) or tumor necrosis factor (x (TNF-alpha). ARPE-19 cells were cultured to confluence. Berberrubine and IL-1 beta or TNF-a were added to the medium. IL-8 and MCP-1 protein concentrations were measured using enzyme-linked immunosorbent assay. IL-8 and MCP-1 mRNA were measured by real time polymerase chain reaction. Nuclear factor kappa B (NF-kappa B) translocation was examined by immunofluorescent staining/microscopy. Berberrubine dose-dependently inhibited IL-8 and NICP- I protein levels in the media and mRNA expression of the cells stimulated with IL-1 beta or TNF-alpha. Immunofluorescent staining/microscopy of NF-kappa B in the nucleus of unstimulated cells was faint (51 14 arbitrary units). Fluorescein was dense (215 42 or 170 24 arbitrary units, respectively) 30 min after stimulation with IL-1 beta or TNF-alpha and was decreased to 62 18 or 47 16 arbitrary units, respectively, by berberrubine. Berberrubine dose-dependently inhibited IL-8 and MCP-1 expression and protein secretion induced by IL-1 beta or TNF-alpha. Possibly, the effect on chemotactic factors may be via suppression of NF-kappa B translocation. (c) 2006 Elsevier Inc. All rights reserved.
机译：我们检查了原小ber碱生物碱小ber红素对白介素-1刺激的人视网膜色素上皮细胞系（ARPE-19）中白介素8（IL-8）和单核细胞趋化蛋白-1（MCP-1）表达的影响。 β（IL-1 beta）或肿瘤坏死因子（x（TNF-alpha）。培养ARPE-19细胞至汇合，将小ber红素和IL-1 beta或TNF-a加入培养基中，将IL-8和MCP-用酶联免疫吸附法测定1种蛋白质的浓度，通过实时聚合酶链反应测定IL-8和MCP-1的mRNA，通过免疫荧光染色/显微镜检查核因子κB（NF-κB）的转运，小Ber碱剂量IL-1β或TNF-α刺激的细胞中介导的IL-8和NICP- I蛋白水平和细胞mRNA表达受到抑制。未刺激细胞核中NF-κB的免疫荧光染色/显微镜观察（ 51 14任意单位）荧光素致密（215 42或170 24任意） IL-1β或TNF-α刺激30分钟后，小红素分别降至62 18或47 16个任意单位。小ber红素剂量依赖性地抑制IL-1β或TNF-α诱导的IL-8和MCP-1表达以及蛋白质分泌。对趋化因子的影响可能是通过抑制NF-κB转运。 （c）2006 Elsevier Inc.保留所有权利。

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